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T3405

Sigma-Aldrich

3,3′,5,5′-Tetramethylbenzidine dihydrochloride

chromogenic, tablet

Sinónimos:

4,4′-Diamino-3,3′,5,5′-tetramethylbiphenyl dihydrochloride

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About This Item

Fórmula empírica (notación de Hill):
C16H20N2 · 2HCl
Número de CAS:
64285-73-0
Peso molecular:
313.27
Número CE:
264-769-6
Número MDL:
MFCD00012961
Código UNSPSC:
12352204
ID de la sustancia en PubChem:
24900140
NACRES:
NA.83

Nombre del producto

3,3′,5,5′-Tetramethylbenzidine dihydrochloride, tablet, 1 mg substrate per tablet

Formulario

tablet

mp

≥300 °C

temp. de almacenamiento

2-8°C

cadena SMILES

Cl[H].Cl[H].Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2

InChI

1S/C16H20N2.2ClH/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14;;/h5-8H,17-18H2,1-4H3;2*1H

Clave InChI

NYNRGZULARUZCC-UHFFFAOYSA-N

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Aplicación

3,3′,5,5′-Tetramethylbenzidine (TMB) is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm.
3,3′,5,5′-Tetramethylbenzidine dihydrochloride has been used as a substrate for the
  • anti-mouse IgG (? specific) horseradish peroxidase-conjugated antibody in the ELISA of mice sera
  • anti-mouse peroxidase antibody in the protein tyrosine phosphatase assay of naive and effector T cell lysate
  • in enzymatic hydroperoxide (EH) assay and in prooxidants–antioxidants balance (PAB) assay of serum samples from diabetic patients

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number
0000408058
0000408058
0000379104
0000320659
0000366382

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Shurong Tang et al.
Mikrochimica acta, 185(5), 273-273 (2018-05-01)
A hybrid material composed of graphitic carbon nitride (g-C3N4) and platinum nanoparticles (PtNPs) with peroxidase mimicking activity was used to design a rapid, sensitive and low-cost colorimetric method for the determination of Ag(I). The g-C3N4-PtNPs hybrid was synthesized by reduction
Sandra Oloketuyi et al.
Biosensors, 11(12) (2021-12-24)
C-reactive protein (CRP) is an inflammation biomarker that should be quantified accurately during infections and healing processes. Nanobodies are good candidates to replace conventional antibodies in immunodiagnostics due to their inexpensive production, simple engineering, and the possibility to obtain higher
CD45 isoform expression is associated with different susceptibilities of human naive and effector CD4+ T cells to respond to IL-4
Stutz A, et al.
European Journal of Immunology, 35(2), 575-583 (2005)
A novel assay for the evaluation of the prooxidant-antioxidant balance, before and after antioxidant vitamin administration in type II diabetes patients
Alamdari DH, et al.
Clinical Biochemistry, 40(3-4), 248-254 (2007)
Darcy S O Mora et al.
Fish & shellfish immunology, 71, 255-263 (2017-09-04)
An experimental contraceptive vaccine was evaluated in Atlantic salmon (Salmo salar). A peptide derived from the beta subunit of luteinizing hormone (LH) was conjugated to two different carrier proteins, bovine serum albumin (BSA) and keyhole limpet hemocyanin (KLH), and formulated
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Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

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