SRP0291

Cathepsin L Active human

recombinant, expressed in FreeStyle^™ 293-F cells, ≥90% (SDS-PAGE)

• Sinónimos: CATL, Major excreted protein (MEP)
• UNSPSC Code: 12352204
• NACRES: NA.54

Propiedades

• biological source: human
• recombinant: expressed in FreeStyle^™ 293-F cells
• assay: ≥90% (SDS-PAGE)
• form: aqueous solution
• specific activity: ≥3900 pmol/min-μg
• mol wt: 36 kDa
• technique(s): inhibition assay: suitable
• suitability: suitable for molecular biology
• NCBI accession no.: NM_001912
• application(s): life science and biopharma
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... CTSL(1514)

Descripción

General description

Research area: Cell Signaling

Cathepsin L is a papain-like cysteine protease and belongs to the Clan A, Family C1. It is composed of L domain of α-helix and an R domain of β-sheet in the spatial structure.

Human cathepsin L (GenBank Accession No. NM_001912), amino acids 18-333, with C-terminal HIS tag, MW = 36 kDa, expressed in FreeStyle 293-F cells.

Application

Active human cathepsin L is useful for the activation of DPP1 (Cathepsin C). Active human cathepsin L has been used:

• to determine that N-acyl and N-sulfonyloxazolidine-2,4-diones are pseudo-irreversible inhibitors of serine proteases
• to investigate the role of cathepsin B and L activity in the serum during the human aging process.
• in inhibitory activity assay
• to study the role of CTSL in COVID-19 infection

Biochem/physiol Actions

Cathepsin L is a cysteine protease, involved primarily in protein breakdown in the lysosome. Cathepsin L degrades nuclear transcription factors and may affect cell cycle regulation. Cathepsin L is also involved in the immune system, specifically in degrading the invariant chain during major histocompatibility complex (MHC) class II processing, a crucial step in antigen presentation. Its expression in the thymus has been demonstrated to be vital for the development of natural killer cells. Additionally, Cathepsin L contributes to recycling processes during axon outgrowth and synapse formation in the developing postnatal central nervous system. Proteases are associated with the development and progression of cancer. Therefore, cathepsin L may be a potential therapeutic target in cancer treatment.

Unit Definition

One unit is defined as the amount of enzyme that will cleave 1 pmol of substrate per min at 37°C

Physical form

Formulated in 50 mM MES, 400 mM NaCl, pH 5.0, and 10% glycerol.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Legal Information

FreeStyle is a trademark of Invitrogen Corp.

Información de seguridad

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable