SAE0049

Lactic Dehydrogenase, recombinant

from human, recombinant, expressed in E. coli, aqueous solution

• Sinónimos: (S)-Lactate: NAD⁺ oxidoreductase, L-Lactate Dehydrogenase
• Número de CAS: 9001-60-9
• Comisión internacional de enzimas: 1.1.1.27 (BRENDA, IUBMB)
• Número MDL: MFCD00131466
• Código UNSPSC: 12352200
• NACRES: NA.54

Propiedades

• origen biológico: human
• Nivel de calidad: 200
• recombinante: expressed in E. coli
• Formulario: aqueous solution
• condiciones de almacenamiento: (Keep container tightly closed in a dry and well-ventilated place)
• color: colorless
• Nº de acceso UniProt: K1T0A2
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C
• Información sobre el gen: human ... LDHA(3939)

Descripción

Descripción general

Research area: Cell Signaling
The gene LDHA (L-lactate dehydrogenase A chain) is mapped to human chromosome 11p15. It is a subunit of lactate dehydrogenase.In particular, lactic dehydrogenase A (LDHA) is mainly found in skeletal muscle, and for that reason is known as the M subunit. This recombinant form of LDHA has a C-terminal histidine-tag.

Aplicación

L-Lactate Dehydrogenase (LDHA) has been used in in vitro phosphoglycerate mutase 1 (PGAM1) inhibitors screening assay. It has also been used in a colorimetric assay for determining lactate concentration in conditioned media.

Acciones bioquímicas o fisiológicas

L-lactic dehydrogenase catalyzes the conversion of L-lactate into L-pyruvate while reducing NAD⁺ to NADH and H⁺.  L-lactate dehydrogenase A chain (LDHA), an enzyme involved in pyruvate metabolism, LDH is responsible for the conversion of pyruvate to lactate, the final step of glycolysis. It is overexpressed in various cancers. LDHA regulates the microenvironment of developing tumors by the hypoxia-inducible factor (HIF)-signaling pathway. LDHA aids in the NAD⁺ regeneration during the β-oxidation of fatty acid. LDHA (L-lactate dehydrogenase A chain) is responsible for the conversion of pyruvate to lactate, the final step of glycolysis. It is overexpressed in various cancers. In cancer cells, HIF-1a (hypoxia-inducible factor) induces the expression of LDHA, which helps in maintaining glycolysis in cells.

L-lactic dehydrogenase catalyzes the conversion of L-lactate into L-pyruvate while reducing NAD⁺ to NADH and H⁺.

Definición de unidad

One unit will reduce 1.0 μmole of pyruvate to L-lactate per min at pH 7.5 at 37 °C.

Forma física

Buffered aqueous solution with Hepes (pH 7.5), NaCl and glycerol.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable