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SAB5700596

Sigma-Aldrich

Recombinant 2019-nCoV Nucleocapsid Protein with His tag

Sinónimos:

Nucleocapsid protein, Nucleoprotein, Protein N

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About This Item

Código UNSPSC:
12352203
NACRES:
NA.26

recombinante

expressed in E. coli

Nivel de calidad

Ensayo

>95% (HPLC)
>95% (SDS-PAGE)

Formulario

liquid

mol peso

49.4 kDa

impurezas

<1.0 EU/μg protein (LAL test)

Nº de acceso UniProt

Condiciones de envío

wet ice

temp. de almacenamiento

−20°C

Información sobre el gen

SARS virus ... N(43740575)

Descripción general

Recombinant SARS-COV-2(2019-nCoV) Nucleocapsid Protein is produced by E. coli expression system. The target protein is expressed with sequence (Met1-Ala419) of SARS-COV-2(2019-nCoV) Nucleocapsid (Accession # QHD43423.2) fused with an 6XHis tag at the N-terminus.

Acciones bioquímicas o fisiológicas

Measured by its binding ability in a functional ELISA. Immobilized Recombinant SARS-COV-2 Nucleocapsid at 2μg/mL (100 μL/well) can bind Anti-Nucleocapsid antibody with a linear range of 0.12-1.64 ng/mL. Measured by its binding ability in a functional ELISA. Immobilized Recombinant SARS-CoV-2 envelope Protein at 2μg/mL (100 μL/well) can bind Recombinant SARS-CoV-2 Nucleocapsid protein, the EC50 of Recombinant SARS-CoV-2 Nucleocapsid protein is 41.10 ng/mL.

Reconstitución

For lyophilized protein : Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.

Almacenamiento y estabilidad

Store the lyophilized protein at -20°C to -80°C for long term. After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8 °C for up to 1 week. This product is stable at ≤ -70°C for up to 6 months from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Data presented is the available current product information and provided as-is. These products have not been tested or verified in any additional applications, sample types, including any clinical use. Experimental conditions must be empirically derived by the user. Our Antibody Guarantee only covers tested applications stated herein and conditions presented in our product information and is not extended to publications.

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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