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Merck
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Key Documents

S9514

Sigma-Aldrich

Superose® 12 Prep Grade

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About This Item

Número de CAS:
97599-42-3
MDL number:
MFCD00166679
UNSPSC Code:
23151817
NACRES:
NA.56

form

suspension

Quality Level

100

particle size

20-40 μm (wet)

pore size

1,000-300,000 Da fractionation range (globular proteins)

storage temp.

2-8°C

Application

Superose® 12 prep grade is used for protein chromatography, gel filtration chromatography and gel filtration media. Superose® 12 prep grade has been used to purify and characterize a haemolysin of Actinomyces pyogenes as well as a fibrinogenase from Vipera lebetina (desert adder) venom. Superose® 12 prep grade has also been used for the isolation and characterization of an extracellular protease of Actinomyces pyogenes.

Other Notes

Highly cross-linked beaded agarose

Physical form

Suspension in 20% ethanol
aqueous ethanol suspension

Legal Information

Superose is a registered trademark of Cytiva

Pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

Hazard Classifications

Flam. Liq. 3

Storage Class

3 - Flammable liquids

wgk_germany

WGK 3

flash_point_f

100.4 - 109.4 °F

flash_point_c

38 - 43 °C

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Sepharose™ CL-4B Cross-linked

Supelco

CL4B200

Sepharose™ CL-4B

Fibrinogen from human plasma 50-70% protein (≥80% of protein is clottable)

Sigma-Aldrich

F3879

Fibrinogen from human plasma

H Youn et al.
Biochimica et biophysica acta, 1388(2), 405-418 (1998-12-22)
Lipoamide dehydrogenase was purified around 22-fold relative to the crude extracts of Streptomyces seoulensis with an overall yield of 9. 5%. The enzyme was composed of two identical subunits with a molecular mass of 54 kDa and contained 1 mol
A Gasmi et al.
Toxicon : official journal of the International Society on Toxinology, 29(7), 827-836 (1991-01-01)
A fibrinogenase from Vipera lebetina venom was isolated by gel filtration in a Superose 12 column prep grade HR 16/50 and by ion-exchange in a Mono Q HR 5/5 column. The purified enzyme, which was obtained with a yield of
G A Ameer et al.
Kidney international, 59(4), 1544-1550 (2001-03-22)
High plasma levels of beta2-microglobulin (beta2m) have been implicated in the formation of the severely destructive and potentially fatal amyloid deposits that are characteristic of dialysis-related amyloidosis (DRA). Conventional renal replacement technologies remove insufficient quantities of beta2m to normalize plasma
Shih-Chieh Lee et al.
Journal of agricultural and food chemistry, 52(16), 4948-4952 (2004-08-05)
Our research on several proteins indicates that accurate molecular weights cannot be determined by Superose 12 column chromatography. In support of this statement, we present data on molecular weights of purified red kidney bean alpha-amylase inhibitor (RKB alphaAI) and white
H Ding et al.
Zentralblatt fur Veterinarmedizin. Reihe B. Journal of veterinary medicine. Series B, 43(3), 179-188 (1996-05-01)
A haemolysin produced by Actinomyces pyogenes ATCC 8164 was purified from culture supernatant by ammonium sulphate and polyethylene glycol precipitation, ion-exchange chromatography on DEAE-Sephacel, and fast-protein-liquid-chromatography on Superose 12 prep grade. The purified haemolysin, designated as pyolysin, displayed a single
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Performing an Isolation of Recombinant Protein Complexes

This page provides information about performing an isolation of recombinant protein complexes with different pull-down assays with products from Cytiva.

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