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SYPRO® Ruby Protein Gel Stain

Sinónimos:

SYPRO® dye, protein gel stain

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About This Item

UNSPSC Code:
41105322
NACRES:
NA.32

shelf life

≥6 mo. (when stored at room temperature and protected from light)

technique(s)

protein staining: suitable

fluorescence

λex 280,450 nm; λem 610 nm

General description

SYPRO Ruby protein gel stain is a ready-to-use, ultrasensitive, luminescent stain for the detection of proteins separated by polyacrylamide gel electrophoresis (PAGE). This stain, designed especially for use in 2-D PAGE, has proven to be an excellent choice for 1-D PAGE and isoelectric focusing (IEF) gels as well. SYPRO Ruby protein gel stain attains sensitivity comparable to many silver stain techniques. However, unlike silver staining, the SYPRO Ruby gel stain:
  • uses a simple staining protocol, with no possibility of overstaining
  • delivers a linear quantitation range of over three orders of magnitude
  • shows less protein-to-protein variability
  • stains glycoproteins, lipoproteins, calcium-binding proteins, fibrillar proteins, and other difficult-to-stain proteins
  • will not stain extraneous nucleic acids
  • does not interfere with subsequent analysis of proteins by Edman-based sequencing or mass spectrometry

Application

SYPRO ruby protein gel stain has been used for staining of the proteins after sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE).

Caution

Protect from light.

Legal Information

SYPRO is a registered trademark of Life Technologies

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Filipe Natalio et al.
Cell and tissue research, 339(2), 429-436 (2009-12-17)
Primmorphs (a three-dimensional sponge primary cell culture system) have been revealed to be a cell/tissue nano-factory for the production of tailor-made hybrid nanostructures. Growth of primmorphs is stimulated by the presence of a titanium alkoxide precursor tolerating titania (TiO2) concentrations
Chronic hypoxia alters mitochondrial composition in human macrophages.
Fuhrmann DC, et al.
Biochimica et Biophysica Acta, 1834, 2750-2760 (2013)
Saskia Hutten et al.
Cell reports, 33(12), 108538-108538 (2020-12-29)
Nuclear import receptors, also called importins, mediate nuclear import of proteins and chaperone aggregation-prone cargoes (e.g., neurodegeneration-linked RNA-binding proteins [RBPs]) in the cytoplasm. Importins were identified as modulators of cellular toxicity elicited by arginine-rich dipeptide repeat proteins (DPRs), an aberrant
Jennifer Osten et al.
The Journal of general physiology, 154(10) (2022-09-03)
The β-myosin heavy chain expressed in ventricular myocardium and the myosin heavy chain (MyHC) in slow-twitch skeletal Musculus soleus (M. soleus) type-I fibers are both encoded by MYH7. Thus, these myosin molecules are deemed equivalent. However, some reports suggested variations
Christoph Schröder et al.
Methods in molecular biology (Clifton, N.J.), 785, 203-221 (2011-09-09)
Antibody microarrays are a multiplexing technique for the analyses of hundreds of different analytes in parallel from small sample volumes of few microlitres only. With sensitivities in the picomolar to femtomolar range, they are gaining importance in proteomic analyses. These
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Protein Staining Reagents Selection Guide

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Sigma offers EZBlue™ and ProteoSilver™ reagents for protein visualization, suitable for proteomics and traditional PAGE formats.

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Sigma offers EZBlue™ and ProteoSilver™ reagents for protein visualization, suitable for proteomics and traditional PAGE formats.

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Protocolos

Cell Lysis and Protein Extraction for Western Blotting

Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.

Cell Lysis and Protein Extraction for Western Blotting

Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.

Cell Lysis and Protein Extraction for Western Blotting

Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.

Cell Lysis and Protein Extraction for Western Blotting

Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.

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