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Merck

RAB0216

Sigma-Aldrich

Human HSP70 ELISA Kit

for serum, plasma, cell culture supernatant and urine

Sinónimos:

HSP70-1, Heat shock 70 kDa protein 1A

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About This Item

Código UNSPSC:
41116158
NACRES:
NA.32

reactividad de especies

human

envase

kit of 96 wells (12 strips x 8 wells)

técnicas

ELISA: suitable
capture ELISA: suitable

entrada

sample type plasma
sample type urine
sample type cell culture supernatant(s)
sample type serum

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 2 ng/mL
standard curve range: 2.469-600 ng/mL

método de detección

colorimetric

Condiciones de envío

wet ice

temp. de almacenamiento

−20°C

Información sobre el gen

human ... HSPA4(3308)

Descripción general

The Human HSP70 (heat shock protein 70) ELISA (enzyme-linked immunosorbent assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human HSP70 in serum, plasma, cell culture supernatants and urine. HSP70 is an important ATP-dependent cytosolic chaperone along with Hsp90. Hspa4 aids in the folding of several proteins and helps in protecting against cellular stresses such as rise in temperature. HSP70 maintains the function of the mitotic centrosome to coordinates bipolar mitotic spindle assembly. Hspa4 might compensate the loss of parkin function in mice by protecting the cells against proteolytic and mitochondrial stress. Therefore, it can serve as an important therapeutic agent for parkinson disease. Upregulation of HSPA4 is observed in parkin gene knockout mice. Hspa4 is known to be associated with tumor progression and offers resistance against many therapeutic agents. The gene is upregulated in many different types of cancer.

Inmunógeno

Recombinant Human HSP70

Aplicación

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Otras notas

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Los componentes del kit también están disponibles por separado

Referencia del producto
Descripción
SDS

  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)SDS

  • RABELADCELISA 1X Assay/Sample Diluent Buffer C (Item L)SDS

  • RABSTOP3ELISA Stop Solution (Item I)SDS

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS

  • RABWASH420X Wash Buffer (Item B)SDS

Pictogramas

Corrosion

Palabra de señalización

Warning

Frases de peligro

Consejos de prudencia

Clasificaciones de peligro

Met. Corr. 1

Código de clase de almacenamiento

8A - Combustible corrosive hazardous materials


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Visite la Librería de documentos

Bing Leng et al.
Neuroscience letters, 723, 134866-134866 (2020-02-29)
Cognitive decline is a common non-motor symptom of Parkinson disease (PD), and cellular prion protein (PrPC) has been suggested to play a role in this process. This study aimed to investigate the correlation between plasma exosomal prion protein and cognitive
Jie Hong et al.
Biological trace element research, 159(1-3), 269-277 (2014-04-17)
The widespread application of lanthanoids (Lns) in manufacturing industries has raised occupational and environmental health concerns about the possible increased health risks to humans exposed to Lns in their working and living environments. Numerous studies have shown that exposures to
Daniel Alcolea et al.
Neurology, 89(2), 178-188 (2017-06-09)
To analyze the clinical utility of 3 CSF biomarkers and their structural imaging correlates in a large cohort of patients with different dementia and parkinsonian syndromes within the spectrum of frontotemporal lobar degeneration (FTLD). We analyzed 3 CSF biomarkers (YKL-40
Maria Augustyniak et al.
Ecotoxicology (London, England), 26(10), 1408-1418 (2017-10-24)
Long lasting exposure of animals to stressing factor may lead to the selection of population able to cope with the stressor at lower cost than unexposed individuals. The aim of this study was to assess whether 130-generational selection of a
Berrak Guven et al.
Regulatory toxicology and pharmacology : RTP, 104, 128-132 (2019-03-18)
Radiation induced colitis is one the most common clinical issue for patients receiving radiotherapy. For this reason, we aimed to investigate the effect of antioxidant-effective flavonoids hesperidin and quercetin on the intestinal damage induced by radiation in this study. TNF-alpha

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