R3654

Anti-RbAp48 antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• Sinónimos: Anti-Retinoblastoma-binding protein
• MDL number: MFCD02686588
• UNSPSC Code: 12352203
• NACRES: NA.41

Propiedades

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen 48 kDa
• species reactivity: human
• technique(s): indirect immunofluorescence: 1-2 μg/mL using HeLa cells fixed with paraformaldehyde-Triton; western blot: 0.5-1 μg/mL using MCF7 nuclear extracts
• UniProt accession no.: Q09028
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... RBBP4(5928) ; mouse ... Rbbp4(19646) ; rat ... Rbbp4(313048)

Descripción

General description

Histone binding protein (RbAp48, also known as also known as RBBP4 or NURF55) contains 425 amino acids, tryptophan-aspartic acid (WD)-domain containing protein isolated as a retinoblastoma binding protein (RB1).

Immunogen

synthetic peptide corresponding to amino acids 1-14 of human RbAp48, conjugated to KLH via a C-terminal added cysteine residue. The immunizing peptide is conserved in human, mouse, and rat.

Application

Anti-RbAp48 antibody produced in rabbit has been used in immunoprecipitation and western blot analysis. It may also be used in immunofluorescence.

Biochem/physiol Actions

RbAp48 is found in association with chromatin remodeling complexes Sin3A/histone deacetylase (HDAC) and NURD (nucleosomal remodeling and deacetylation complex).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Información de seguridad

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)