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R0503

Sigma-Aldrich

Reactive Red 120−Agarose

saline suspension, Type 3000-CL

Sinónimos:

Reactive Red Agarose, Red 120-Agarose, Red Agarose

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About This Item

MDL number:
MFCD00165711
UNSPSC Code:
23151817
NACRES:
NA.56

biological source

plant

Quality Level

200

type

Type 3000-CL

form

saline suspension

extent of labeling

≥3 μmol per mL

technique(s)

affinity chromatography: suitable

matrix

cross-linked 4% beaded agarose

suitability

suitable for chromatography

storage temp.

2-8°C

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Application

Reactive Red 120-agarose is used in affinity chromatography, protein chromatography and dye resins. Reactive Red 120-agarose has been used to study wheat quality breeding as well as to provide strong evidence that purified human P-glycoprotein (Pgp) functions as an ATP-dependent drug transporter.

Physical form

Suspension in 0.5 M NaCl containing preservative

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Koji Iwamoto et al.
Plant physiology, 133(2), 893-900 (2003-09-16)
Mannitol-1-phosphate (M1P) dehydrogenase (M1PDH; EC 1.1.1.17), an enzyme catalyzing the reduction of Fru-6-phosphate (F6P) to M1P in algal mannitol biosynthesis, was purified to homogeneity from a cell homogenate of the eulittoral red alga Caloglossa continua (Okamura) King et Puttock. The
P De Prada et al.
Applied and environmental microbiology, 63(7), 2928-2931 (1997-07-01)
Two extracellular, heat-labile alkaline phosphatases were purified from a psychrophilic Arthrobacter isolate, D10. The enzymes were active over different pH ranges, used distinct substrates, and had different kinetic properties. Each enzyme reacted specifically to its own antibody during immunoblot analysis.
Removing trace fluorescent contaminants from GroEL preparations.
F Weber
Methods in molecular biology (Clifton, N.J.), 140, 63-64 (2001-08-04)
A Y Strongin et al.
The Journal of biological chemistry, 270(10), 5331-5338 (1995-03-10)
Matrix metalloproteases are secreted by mammalian cells as zymogens and, upon activation, initiate tissue remodeling by proteolytic degradation of collagens and proteoglycans. Activation of the secreted proenzymes and interaction with their specific inhibitors determine the net enzymatic activity in the
S V Ambudkar et al.
Methods in enzymology, 292, 492-504 (1998-08-26)
Human Pgp from the vinblastine-resistant cell line, KB-V1, can be purified by sequential conventional chromatography on DEAE-sepharose CL-6B resin followed by a wheat germ agglutinin column. By including glycerol (osmolyte protectant) and lipid during the solubilization and chromatography procedures most
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