PIC0004

Protease Inhibitor Cocktail

ReadyShield^® ready-to-use solution, for the inhibition of serine, cysteine, acid proteases and aminopeptidases, for use in purification of Histidine-tagged proteins, Non-freezing solution

• Sinónimos: Protease inhibitor cocktail
• UNSPSC Code: 12352200
• NACRES: NA.28
• Form: ready-to-use solution
• Solubility: water: soluble
• Storage temp.: −20°C

Propiedades

• Nombre del producto: ReadyShield^® Protease Inhibitor Cocktail, for use in purification of Histidine-tagged proteins, Non-freezing solution
• description: Purification of Histidine-Tagged Proteins
• Quality Segment: 100
• form: ready-to-use solution
• quality: 200
• packaging: pkg of 1 mL (in amber polypropylene vial), pkg of 5 mL (in amber polypropylene vial)
• input: general use sample(s)
• solubility: water: soluble
• storage temp.: −20°C

Descripción

General description

This particular protease inhibitor cocktail omits chelators (e.g., EDTA), to optimize its use for purification of histidine-tagged proteins.1 It contains inhibitors with a broad specificity for serine, cysteine, and acid proteases, and aminopeptidases. The cocktail has been tested on extracts from E. coli and insect cells This cocktail is supplied as a ready-to-use solution using a proprietary non-freezing formulation. ReadyShield PIC0004 is a non-freezing formulation that contains the same inhibitors as is the DMSO based cocktail P8849.

Features and Benefits

• Does not freeze in storage for quick, convenient use.
• Tailored for use with Histidine fusion tagged proteins.
• Suggested as 100 X solution.
• Reclosable screw-top vials.
• Can be stored in cooler for approx. 1 month or room temperature for a week.

Preparation Note

The recommended dilution of the cocktail in the biological extract is 1 ml of the cocktail per 100 ml of lysate from 20 g (wet weight) of E. coli cells or 10 g (wet weight) of baculovirus-infected insect (Spodoptera frugiperda) cells.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

ReadyShield is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• pictograms: GHS07
• signalword: Warning
• hcodes: H315,H319
• pcodes: P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313 - P337 + P313
• Hazard Classifications: Eye Irrit. 2 - Skin Irrit. 2
• Clase de almacenamiento: 10 - Combustible liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable