The protein content and enzymatic activity of this product vary from lot to lot. The protein content will range from 20% - 40%, and the activity will range from 10 units - 50 units per milligram of protein. As an example, a batch that reports a protein content of 20% and an activity of 10 units per milligram of protein will have a vial content of 12.5 mg for the 25 unit package size. The protein content and activity are reported in the product Certificate of Analysis. Please see the link below to review a sample or lot specific Certificate:
https://www.sigmaaldrich.com/product/sigma/p7633#product-documentation
P7633
Phospholipase C from Clostridium perfringens (C. welchii)
Type I, lyophilized powder, 10-50 units/mg protein
Sinónimos:
PC-PLC
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About This Item
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Productos recomendados
origen biológico
bacterial (Clostridium perfringens)
Nivel de calidad
tpo
Type I
Formulario
lyophilized powder
actividad específica
10-50 units/mg protein
composición
protein, 20-40%
características de los productos alternativos más sostenibles
Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
aplicaciones
diagnostic assay manufacturing
categoría alternativa más sostenible
, Enabling
temp. de almacenamiento
−20°C
Información sobre el gen
Clostridium perfringens str. 13 ... plc(988262)
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Categorías relacionadas
Descripción general
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in biodiesel research. For more information see the article in biofiles.
Aplicación
Phospholipase C (PLC) has been used to study adrenoceptor-mediated transmembrane signaling. It is also used to degrade inositol-containing phospholipids. Furthermore, it has been used to study 9E3 gene activation[1].
Acciones bioquímicas o fisiológicas
Hydrolyzes the phosphate bond on phosphatidylcholine and other glycerophospholipids yielding diacylglycerol; this enzyme will also hydrolyze the phosphate bonds of sphingomyelin, cardiolipin, choline plasmalogen and ceramide phospholipids.
PLC hydrolyzes the phosphate bond on phosphatidylcholine and other glycerophospholipids yielding diacylglycerol. This enzyme also hydrolyzes the phosphate bonds of sphingomyelin, cardiolipin, choline plasmalogen and ceramide phospholipids. Phospholipase C is induced by thrombin and platelet-activating factor, forming 1,2-diacylglycerol and phosphatidic acid.
Definición de unidad
One unit will liberate 1.0 μmole of water soluble organic phosphorus from egg yolk L-α-phosphatidylcholine per min at pH 7.3 at 37 °C.
Inhibidor
Referencia del producto
Descripción
Precios
sustrato
Referencia del producto
Descripción
Precios
Palabra de señalización
Danger
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Resp. Sens. 1
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Equipo de protección personal
Eyeshields, Gloves, type N95 (US)
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Certificados de análisis (COA)
Lot/Batch Number
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Los clientes también vieron
R Spangler et al.
Proceedings of the National Academy of Sciences of the United States of America, 86(18), 7017-7021 (1989-09-01)
Induction of the transformation-related gene 9E3 by the v-src and v-fps gene products (v-Src and v-Fps) is blocked in chicken embryo fibroblasts depleted of protein kinase C (PKC). PKC agonists induce 9E3 gene expression. Protein kinase inhibitors block v-Src- and
Mojtaba Alimolaei et al.
Probiotics and antimicrobial proteins, 10(2), 251-257 (2017-04-13)
The alpha-toxin is one of the virulence factors of Clostridium perfringens for gas gangrene in humans and animals or necrotic enteritis in poultry. The C-terminal domain of this toxin ( cpa 247-370 ) was synthesized and cloned into pT1NX vector
Sam Behjati et al.
Nature genetics, 46(4), 376-379 (2014-03-19)
Angiosarcoma is an aggressive malignancy that arises spontaneously or secondarily to ionizing radiation or chronic lymphoedema. Previous work has identified aberrant angiogenesis, including occasional somatic mutations in angiogenesis signaling genes, as a key driver of angiosarcoma. Here we employed whole-genome
C Little et al.
Biochimica et biophysica acta, 391(2), 326-333 (1975-06-24)
1. The zinc content and metal ion dependence of phospholipase C(phosphatidylcholine cholinephosphohydrolase, EC 3.1.4.3) from Bacillus cereus have been examined. 2. The native enzyme contained about 2 atoms of tightly bound zinc/molecule. 3. Incubation of the enzyme with EDTA or
Zahra Timsah et al.
Nature structural & molecular biology, 21(2), 180-188 (2014-01-21)
FGFR2-expressing human cancer cells with low concentrations of the adaptor protein Grb2 show high prevalence for metastatic outcome. In nonstimulated cells, the SH3 domain (and not the SH2 domains) of Plcγ1 directly competes for a binding site at the very
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what is the meaning of 10-50 units/mg protein.
1 answer-
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P7633とP4039の精製方法の概要（方法名のみで差し支えございません）を開示して頂くことは可能でしょうか？
1 answer-
Unfortunately, the purification method for these products are considered proprietary. However, the following reference may be of interest:
Purification of Clostridium perfringens phospholipase C (alpha-toxin) by affinity chromatography on agarose-linked egg-yolk lipoprotein. T Takahashi et al. Biochimica et biophysica acta, 351(1), 155-171 (1974-05-10)
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What are the differences in the purification methods between Products P7633 and P4039 Phospholipase C from Clostridium perfringens (C. welchii)?
1 answer-
The extraction and purification methods for our Phospholipase C products are proprietary, however higher unit per milligram values indicate a more purified product. Product P7633 contains a specific activity of 10-50 units/mg protein and the protein content is between 20.0 - 40.0 %. Product P4039 will contain a specific activity of ≥150 units/mg protein. The protein content for this product is between 5.0 - 30.0 %. The impact of the methods on the isoform profile of these products is not evaluated. The specific activity of each lot activity will be reported in the Certificate of Analysis. Please view the sample Certificate of Analysis:
https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/P7633-500UN0000218762.pdf
https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/P4039-125UN0000185061.pdfHelpful?
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
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Are Products P7633 and P4039, Phospholipase C from Clostridium perfringens (C. welchii), prepared from the same strain?
1 answer-
Products P7633 and P4039, Phospholipase C, from Clostridium perfringens (C. welchii) are prepared from the same strain.
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