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P5985

Sigma-Aldrich

Protease from Bacillus sp.

liquid, ≥16 U/g

Sinónimos:

Everlase 16.0 L

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About This Item

Número CE:
Número MDL:
Código UNSPSC:
12352204
NACRES:
NA.54

origen biológico

Bacillus sp.

Nivel de calidad

Formulario

liquid

actividad específica

≥16 U/g

mol peso

20--30 kDa

temp. de almacenamiento

2-8°C

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Descripción general

Proteases are ubiquitously found in nature and microbes are the desired source for these enzymes. They are classified into four classes namely aspartic, serine, cysteine and metalloproteases.

Aplicación

Protease from Bacillus sp. has been used to determine its proteolytic activity by spectrophotometric method.

Acciones bioquímicas o fisiológicas

Protease catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphate. Proteases, secreted from Bacillus sp., typically have molecular weights ranging from 20,000 to 30,000. They are typically stabilized by Ca2+ and have high isoelectric points. It is highly soluble in detergents at a wide range of pH and temperatures. Protease improves healing process by efficiently removing necrotic materials from wounds. It is extensively used in laundry detergents to remove protein based stains from clothing. In addition, protease is also used in food, pharmaceutical, leather and silk industries. It is a key constituent in biopharmaceutical products including contact-lens enzyme cleaners and enzymatic deriders. Proteases also aid in various physiological processes such as protein turnover, digestion, blood coagulation, fertilization and cell differentiation. It also facilitates growth, cell signaling, the immune response, and apoptosis.

Información legal

A product of Novozyme Corp.

Pictogramas

Health hazardCorrosion

Palabra de señalización

Danger

Frases de peligro

Clasificaciones de peligro

Aquatic Chronic 3 - Eye Dam. 1 - Resp. Sens. 1

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 2

Equipo de protección personal

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


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Isolation, production and characterization of protease from Bacillus sp. isolated from soil sample
Josephine FS, et al.
Journal of Microbiology, 2(1), 163-168 (2012)
Irreversible inhibitors of serine, cysteine, and threonine proteases
Powers JC, et al.
Chemical Reviews, 102(12), 4639-4750 (2002)
Effect of UV light on microbial proteases: From enzyme inactivation to antioxidant mitigation.
Lante A, et al.
Innovative Food Science & Emerging Technologies, 17, 130-134 (2013)
Laure El Chamy et al.
Nature immunology, 9(10), 1165-1170 (2008-08-30)
In drosophila, molecular determinants from fungi and Gram-positive bacteria are detected by circulating pattern-recognition receptors. Published findings suggest that such pattern-recognition receptors activate as-yet-unidentified serine-protease cascades that culminate in the cleavage of Spätzle, the endogenous Toll receptor ligand, and trigger
Sara Toja Ortega et al.
Water research X, 16, 100151-100151 (2022-08-16)
In aerobic granular sludge (AGS) reactors, granules of different sizes coexist in a single reactor. Their differences in settling behaviour cause stratification in the settled granule bed. In combination with substrate concentration gradients over the reactor height during the anaerobic

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