NA0200

GenElute^™ HP Plasmid Midiprep Kit

sufficient for 25 purifications

• Sinónimos: GenElute^™ HP Plasmid Midiprep Kit, plasmid extraction kit, plasmid isolation kit, plasmid purification kit, Gen Elute
• NACRES: NA.52
• UNSPSC Code: 41105501

Propiedades

• usage: sufficient for 25 purifications
• Quality Segment: 200
• greener alternative product characteristics: Designing Safer Chemicals; Learn more about the Principles of Green Chemistry.
• sustainability: Greener Alternative Product
• technique(s): DNA purification: suitable
• test parameters: sample volume: 1-5 mL bacterial culture
• greener alternative category: , Aligned
• storage temp.: 15-25°C

Descripción

General description

The GenElute HP Plasmid Midiprep Purification kits offer an ultrafast and efficient solution for large-scale plasmid preparation from E. coli cultures. These kits combine silica-based membrane technology and the convenience of spin or vacuum format. The kits also include a special filter cartridge, which replaces the centrifugation step following alkaline lysis. Following lysis the DNA is bound to the silica membrane and contaminants are removed with a simple wash step. The DNA is then eluted in water or TE buffer by centrifugation.

The purified plasmid DNA is suitable for a wide variety of molecular biology applications, including restriction digestions and sequencing.

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.

Click here to view the kit protocol

Application

GenElute^™ HP Plasmid Midiprep Kit has been used to isolate and purify plasmids.

Features and Benefits

• From harvested bacterial culture to pure plasmid DNA in 30 minutes or less
• Up to 350 μg of high-copy plasmid DNA
• Flexibility of a vacuum or spin format
• No phenol/chloroform extraction or alcohol precipitation required
• Contains fewer plastic components, reducing the amount of waste

Other Notes

For additional information, please see www.sigma-aldrich.com/genelutehp.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Los componentes del kit también están disponibles por separado

• R1149

  Resuspension Solution, Add RNase A before use. After addition of RNAse, store at 2-8ºC. )

  SDS
• W0263

  Wash Solution 1

  SDS
• W4639

  Wash Solution 2

  SDS
• R6148

  RNase A solution

  SDS
• C2112

  Column Preparation Solution

  SDS
• L1912

  Lysis Solution

  SDS

Información de seguridad

• pictograms: GHS02,GHS08,GHS05,GHS07
• signalword: Danger
• pcodes: P210 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• target_organs: Central nervous system
• Clase de almacenamiento: 3 - Flammable liquids
• wgk: WGK 3
• hcodes: H226,H290,H302 + H332,H314,H334,H336
• Hazard Classifications: Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Corr. 1B - STOT SE 3