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Merck

MBD0055

Sigma-Aldrich

MediaBoost

non-animal sterile microbial growth supplement

Sinónimos:

growth supplement for germination, microbial culture additive, microbial culture enhancer, microbial media supplement, minimal growth media supplement

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About This Item

UNSPSC Code:
41106212
NACRES:
NB.21

Quality Level

sterility

sterile

form

liquid

technique(s)

microbiological culture: suitable

shipped in

wet ice

storage temp.

2-8°C

suitability

Bacillus spp.
Escherichia spp.
Lactobacillus spp.
Lactococcus spp.
Pseudomonas spp.
Saccharomyces spp.
bacteria
yeasts

General description

Studying microbial species via cutting edge genomic techniques such as next generation sequencing (NGS) has become increasingly prevalent in recent years; however, genomic methods alone cannot fully replace experimental testing of microbial species. Therefore, the ability to culture microbes in a laboratory setting remains essential for comprehensive study of these communities. Some microbial species can be challenging to culture in standard culture media. Often species of interest or a specific process require specialized media compositions, to provide an optimal environment for maximal proliferation and yield. A few examples includes

  • Chemically defined minimal growth media are often used to grow bacteria for proteomics, as a base for stable isotope enrichment in NMR protein structure-function studies, but growth can be limited without the addition of an effective supplement.

  • Use of chemically defined minimal growth media in the production of secreted microbial proteins to ensure reproducibility and a higher concentration of the microbial-produced protein of interest.

Application

MediaBoost provides microbial researchers a ready-to-use media supplement to amplify growth in selected gram-positive bacteria, gram-negative bacteria, and yeast species. Application data is available for some of the strains and media combinations that have been tested with MediaBoost, including:
  • Bacillus subtilis spores in LB medium
  • Lactobacillus rhamnosus in LB medium
  • Pseudomonas aeruginosa in M9 medium
  • Saccharomyces cerevisiae in YPD, BM2, and IMDM media

Please note strains and media combinations and should be tested on a case-by-case basis by the researcher for experiment optimization.

Features and Benefits

  • Boosts Growth of Certain Microorganisms
  • Reduces Germination Time
  • Ready-Made, Sterile Solution
  • Protein-Free

related product

Referencia del producto
Descripción
Precios

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Chandar S Thakur et al.
Applied microbiology and biotechnology, 88(3), 771-779 (2010-08-24)
Since RNAs lie at the center of most cellular processes, there is a need for synthesizing large amounts of RNAs made from stable isotope-labeled nucleotides to advance the study of their structure and dynamics by nuclear magnetic resonance (NMR) spectroscopy.
Maryam Tidjani Alou et al.
Clinical microbiology reviews, 34(1) (2020-10-30)
The last 5 years have seen a turning point in the study of the gut microbiota with a rebirth of culture-dependent approaches to study the gut microbiota. High-throughput methods have been developed to study bacterial diversity with culture conditions aimed
Anna Gąciarz et al.
Microbial cell factories, 16(1), 108-108 (2017-06-18)
The production of recombinant proteins containing disulfide bonds in Escherichia coli is challenging. In most cases the protein of interest needs to be either targeted to the oxidizing periplasm or expressed in the cytoplasm in the form of inclusion bodies
Stephan B Azatian et al.
Journal of biomolecular NMR, 73(1-2), 11-17 (2019-01-08)
We describe a general and simple modification to the standard M9 minimal medium recipe that leads to an approximate twofold increase in the yield of heterologously expressed proteins in Escherichia coli BL21(DE3) bacteria. We monitored the growth of bacteria transformed
Chi-Ling Tseng et al.
Applied microbiology and biotechnology, 93(4), 1539-1552 (2011-08-09)
Bacterial lipoproteins are crucial antigens for protective immunity against bacterial pathogens. Expression of exogenous lipoproteins in Escherichia coli at high levels is thought to be an extremely difficult endeavor because it frequently results in incomplete or absent lipid modification. Previously

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