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MAK196

Sigma-Aldrich

Malate Dehydrogenase Assay Kit

Sufficient for 100 Colorimetric tests

Sinónimos:

MDH Activity Assay Kit

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About This Item

Código UNSPSC:
12161503
NACRES:
NA.84

método de detección

colorimetric

enfermedades relevantes

neurological disorders

temp. de almacenamiento

−20°C

Información sobre el gen

human ... MDH1(4190) , MDH1B(130752) , MDH2(4191)
mouse ... MDH1(17449) , MDH1B(76668) , MDH2(17448)
rat ... MDH1(24551) , MDH1B(316444) , MDH2(81829)

Categorías relacionadas

Descripción general

Malate dehydrogenase (MDH; EC 1.1.1.37) is an NAD oxidoreductase that catalyzes the NAD/NADH-dependent interconversion of malate and oxalacetate. This reaction is critical to maintain the malate/aspartate shuttle across the mitochondrial membrane and Krebs tricarboxylic acid cycle in the mitochondrial matrix. Increased MDH activity has been observed in neurodegenerative diseases such as Alzheimer′s disease.

Aplicación

Malate Dehydrogenase Assay Kit has been used in LDH (lactate dehydrogenase) and malate dehydrogenase (MDH1) assays. It has also been used to estimate isocitrate dehydrogenase and succinate dehydrogenase activities.

Idoneidad

Suitable for the detection of Malate Dehydrogenase activity in a variety of biological samples such as cell and tissue culture supernatants and purified mitochondria.

Principio

The Malate Dehydrogenase Assay Kit provides a simple and sensitive procedure for measuring MDH activity in a variety of tissues, cell cultures, and isolated mitochondria. MDH activity is determined by generating a product with absorbance at 450 nm proportional to the enzymatic activity present. One unit of MDH is the amount of enzyme that generates 1.0 μmole of NADH per minute at 37 °C and pH 9.5.

Solo componentes del kit

Referencia del producto
Descripción
  • MDH Assay Buffer
  • MDH Substrate
  • MDH Enzyme Mix
  • MDH Developer
  • NADH Standard
  • MDH Positive Control

sustituido por

Referencia del producto
Descripción
Precios

Pictogramas

Corrosion
GHS05

Palabra de señalización

Danger

Frases de peligro

H314

Clasificaciones de peligro

Eye Dam. 1 - Skin Corr. 1B

Código de clase de almacenamiento

8A - Combustible corrosive hazardous materials

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Certificados de análisis (COA)

Lot/Batch Number
5J09K06540
7J16K06540
2L02K06540
B9K110654
B9K050654

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Protocolos

Enzymatic Assay of Malic Dehydrogenase

Spectrophotometric assay evaluates malic dehydrogenase activity using bovine heart enzyme with critical histidine residue at active site.

Enzymatic Assay of Malic Dehydrogenase

Spectrophotometric assay evaluates malic dehydrogenase activity using bovine heart enzyme with critical histidine residue at active site.

Enzymatic Assay of Malic Dehydrogenase

Spectrophotometric assay evaluates malic dehydrogenase activity using bovine heart enzyme with critical histidine residue at active site.

Enzymatic Assay of Malic Dehydrogenase

Spectrophotometric assay evaluates malic dehydrogenase activity using bovine heart enzyme with critical histidine residue at active site.

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