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M8945

Matrix Metalloproteinase-9 human

recombinant, >90% (SDS-PAGE), buffered aqueous solution

Sinónimos:

95 kDa Gelatinase, Gelantinase-B, MMP-9

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UNSPSC Code:
12352204
NACRES:
NA.32
MDL number:
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Quality Segment

assay

>90% (SDS-PAGE)

form

buffered aqueous solution

mol wt

apparent mol wt ~93 kDa

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... MMP9(4318)

Biochem/physiol Actions

MMP-9 degrades the collagens that make up the extracellular matrix, which has a role in the control of angiogenesis.

Physical form

Solution in Tris-HCl, pH 7.5, Calcium Chloride, Sodium Chloride, and Brij-35

Analysis Note

The biological activity is measured by its ability to cleave a fluorogenic peptide substrate.

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Este artículo
SAE0077SAE0078M9070
assay

>90% (SDS-PAGE)

assay

95% (SDS-PAGE)

assay

≥95% (SDS-PAGE)

assay

>90% (SDS-PAGE)

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

form

buffered aqueous solution

form

liquid

form

liquid

form

lyophilized powder

mol wt

apparent mol wt ~93 kDa

mol wt

calculated mol wt 76 kDa, observed mol wt 92 kDa (The protein migrates as a 92 kDa protein on SDS-PAGE due to glycosylation)

mol wt

calculated mol wt 66 kDa, observed mol wt 82 kDa by SDS-PAGE (The protein migrates as a 82 kDa protein on SDS-PAGE due to glycosylation)

mol wt

apparent mol wt ~69 kDa

UniProt accession no.

P14780

UniProt accession no.

P14780

UniProt accession no.

P14780

UniProt accession no.

P08253

Gene Information

human ... MMP9(4318)

Gene Information

human ... MMP(4318)

Gene Information

human ... MMP(4318)

Gene Information

human ... MMP2(4313)


Clase de almacenamiento

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)



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Questions

1–4 of 4 Questions  
  1. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  2. Is product M8945 (Marix Metalloproteinase-9 Human) endotoxin free?

    1 answer
    1. This product contains < 1.0 EU/ug of the enzyme.

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  3. Does product M8945 (Matrix Metalloproteinase-9 Human) need to be activated?

    1 answer
    1. To activate recombinant human MMP-9, prepare a p-aminophenylmercuric acetate (APMA) solution in DMSO. Add the AMPA solution to the MMP-9 to a final APMA concentration of 1 mM. Incubate at 37°C. for 16 - 24 hours.

      Helpful?

  4. What is the molecular weight of product M8945 (Matrix Metalloproteinase-9 Human)

    1 answer
    1. Human MMP-9 consists of a 688 amino acid residue recombinant protein which has a predicted molecular mass of approximately 77 kDa. By SDS-PAGE the apparent molecular mass is approximately 93 kDa.

      Helpful?

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