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Merck

M7150

Sigma-Aldrich

Murashige and Skoog Vitamin Powder

1000 ×, powder, suitable for plant cell culture

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.72
En este momento no podemos mostrarle ni los precios ni la disponibilidad

form

powder

Quality Level

concentration

1000 ×

technique(s)

cell culture | plant: suitable

pH

3.5-4.5 (1.031 g/10 mL)

application(s)

agriculture

shipped in

ambient

storage temp.

2-8°C

Application

Murashige and Skoog medium is a widely used plant tissue culture growth medium. M&S Basal Medium contains macronutrients that include high levels of nitrate and organic additives such as agar, sugars, vitamins and growth regulators. Important growth regulators frequently added to M&S include IAA (auxin/morphogen) and the Kinetin (cytokinin/cell division promoter).

Preparation Note

Murashige and Skoog medium can be reconstituted from powder or by combining products that are major components of complete M&S medium, such as macronutrient mixtures and vitamin mixtures. Murashige and Skoog Vitamin powder (M7150) can be combined with Murashige and Skoog micro/macro-nutrients to generate basal media which when supplemented with organics, agar, auxins and cytokinins yield complete medium for growth plant tissue culture.

Reconstitution

Use at a concentration of one mL per liter of prepared medium to achieve the proper final concentration.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Elsa Matthus et al.
Plant physiology, 179(4), 1754-1767 (2019-01-31)
Phosphate (Pi) deficiency strongly limits plant growth, and plant roots foraging the soil for nutrients need to adapt to optimize Pi uptake. Ca2+ is known to signal in root development and adaptation but has to be tightly controlled, as it
Elizabeth J Carino et al.
Journal of virology, 94(22) (2020-08-28)
Maize chlorotic mottle virus (MCMV) combines with a potyvirus in maize lethal necrosis disease (MLND), a serious emerging disease worldwide. To inform resistance strategies, we characterized the translation initiation mechanism of MCMV. We report that MCMV RNA contains a cap-independent
Saxena et al.
Plant science : an international journal of experimental plant biology, 155(2), 133-140 (2000-05-18)
Efficient protocols have been established for both direct and indirect regeneration of plants in Pelargonium graveolens Indian cultivar Hemanti (Algerian type). Murashige and Skoog's (MS) medium [T. Murashige, F. Skoog, A revised medium for rapid growth and bioassays with tobacco

Questions

1–2 of 2 Questions  
  1. Does this contain agar? And what is the production method?

    1 answer
    1. This product does not contain agar. Please see the link below to review the composition of this item:
      https://www.sigmaaldrich.com/technical-documents/protocol/cell-culture-and-cell-culture-analysis/plant-tissue-culture/vitamin-mixtures

      The method of production is considered proprietary.

      Helpful?

  2. do you have the product information? 

    1 answer
    1. The product detail page contains information such as properties, applications, components, and preparation instructions for this product. From the product detail page DOCUMENTATION section, additional information may be obtained, such as the Certificate of Analysis and SDS. Please see the documents below:

      SDS
      https://www.sigmaaldrich.com/sds/sigma/m3900

      COA
      https://www.sigmaaldrich.com/coa/SIGMA/M3900/RNBL5855

      For more information, we kindly ask you to navigate to the link https://www.sigmaaldrich.com/techservice, and click on "Product Technical Inquires" under the Products Section with all the required information so that a member of our team can reach out to you to assist further. Thank you.

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