The main difference between M4287 and M0503 is that M4287 is specifically recommended for cell culture and is cell culture tested with a recommended concentration for use in eukaryotic cell culture.
M4287
Mitomicina C from Streptomyces caespitosus
powder, suitable for cell culture, BioReagent
Sinónimos:
Mitomycin
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About This Item
Fórmula empírica (notación de Hill):
C15H18N4O5
Número de CAS:
Peso molecular:
334.33
Beilstein/REAXYS Number:
7231816
MDL number:
UNSPSC Code:
12352207
PubChem Substance ID:
NACRES:
NA.76
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Nombre del producto
Mitomicina C from Streptomyces caespitosus, powder, BioReagent, suitable for cell culture
biological source
Streptomyces caespitosus
Quality Level
product line
BioReagent
form
powder
technique(s)
cell culture | mammalian: suitable
color
gray
purple/blue
(1) 3.2, (2) 6.5
solubility
H2O: 4 mL/vial, clear to slightly hazy, blue to purple (Stock solutions should be filter sterilized and stored at 2-8 °C in the dark.)
antibiotic activity spectrum
Gram-negative bacteria
Gram-positive bacteria
mode of action
DNA synthesis | interferes
storage temp.
2-8°C
SMILES string
[H][C@]12CN3C4=C([C@@H](COC(N)=O)[C@@]3(OC)[C@@]1([H])N2)C(=O)C(N)=C(C)C4=O
InChI
1S/C15H18N4O5/c1-5-9(16)12(21)8-6(4-24-14(17)22)15(23-2)13-7(18-13)3-19(15)10(8)11(5)20/h6-7,13,18H,3-4,16H2,1-2H3,(H2,17,22)/t6-,7+,13+,15-/m1/s1
InChI key
NWIBSHFKIJFRCO-WUDYKRTCSA-N
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General description
La mitomicina C es un quimioterápico sistémico y un antibiótico obtenido de Streptomyces caespitosus.[1][2] Es el primer alquilante biorreductor que se identificó y es un derivado del ácido 3-amino-5-hidroxibenzoico (AHBA), la D-glucosamina, la L-metionina y el carbamoilfosfato.[3]
Estructura química: aciridina
Estructura química: aciridina
Application
La mitomicina C de Streptomyces caespitosus se ha utilizado para el tratamiento de capas de células alimenticias, como los PMEF (fibroblastos embrionarios primarios de ratón) y los fibroblastos embrionarios de ratón CD1 (MEF) para el cultivo de hESC (células madre embrionarias humanas).[4][5][6] También se ha utilizado para el tratamiento de la línea celular BLC (cáncer «basal-like»).[7]
Biochem/physiol Actions
La mitomicina C provoca el entrecruzamiento del ADN bicatenario, lo que produce mutagénesis, inhibición de la síntesis de ADN, inicio de eventos de reparación del ADN y activación de la apoptosis. La capacidad para inhibir la síntesis de ADN es atribuible al grupo aminoquinona presente en la mitomicina C.[3][8] Este compuesto no afecta a la síntesis de ARN ni de proteínas. En cultivo de tejidos, este compuesto reduce la viabilidad celular y suprime la mitosis, y causa desorganización del núcleo y la producción de células gigantes.[2] La mitomicina C tiene potentes actividades anti-gramnegativas y anti-grampositivas.[8] Contiene tres restos, los grupos quinina, uretano y aciridina. Se utiliza para generar células nutritivas mitóticamente inactivas en sistemas de cultivo celular, como los fibroblastos mitóticamente inactivos utilizados en los sistemas de células madre embrionarias.
Modo de acción: Este producto es un agente alquilante dirigido específicamente a la secuencia del nucleósido de guanina 5′-CpG-3′. Inhibe la síntesis de ADN mediante reacción covalente con el ADN al establecer enlaces transversales entre las cadenas complementarias del ADN. Esta interacción evita la separación de las cadenas de ADN complementarias, inhibiendo así la replicación del ADN.
Espectro antimicrobiano: La mitomicina C tiene una fuerte actividad antitumoral, en especial contra las células tumorales de la ascitis de Ehrlich, y una fuerte acción bactericida contra las bacterias grampositivas y gramnegativas.
Espectro antimicrobiano: La mitomicina C tiene una fuerte actividad antitumoral, en especial contra las células tumorales de la ascitis de Ehrlich, y una fuerte acción bactericida contra las bacterias grampositivas y gramnegativas.
Caution
Este vial contiene 2 mg de mitomicina C y 48 mg de NaCl. Las disoluciones de reserva deben esterilizarse mediante filtración y conservarse a 2 - 8°C en la oscuridad. Las disoluciones a pH 6-9 pueden conservarse entre 0 y 5°C durante un máximo de una semana, pero si se forma un precipitado, debe prepararse una nueva disolución; se ha demostrado que la disolución precipitada es tóxica para las células.
Preparation Note
La mitomicina C es soluble en agua a 0,5 mg/ml, con un pH de 6,0-7,5. Experimenta degradación rápida en disoluciones ácidas con un pH<6 y es más probable que conserve su actividad en disoluciones con un pH entre 6 y 9.
Related product
Referencia del producto
Descripción
Precios
suggested gloves for splash protection
signalword
Warning
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Carc. 2
Storage Class
13 - Non Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Artículos
Antibiotic kill curve is a dose response experiment in which mammalian cells are subjected to increasing amounts of selection antibiotic
Antibiotic kill curve is a dose response experiment in which mammalian cells are subjected to increasing amounts of selection antibiotic
Antibiotic kill curve is a dose response experiment in which mammalian cells are subjected to increasing amounts of selection antibiotic
Antibiotic kill curve is a dose response experiment in which mammalian cells are subjected to increasing amounts of selection antibiotic
Protocolos
This technical article covers the indirect co-culture of embryonic stem cells with embryonic fibroblasts.
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What is the main difference between M4287 and M0503? And are both of them suitable for cell culture?
1 answer-
Helpful?
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Where can I find out the expiry date of the product (powder form, not dissolved yet)?
1 answer-
This product is not assigned an expiration date. It is assigned a recommended retest date of approximately 4 years after the quality release date. A recommended retest date is the time during which the product is expected to remain within established stability specifications, provided that it has been stored under defined conditions. After the Retest Date, product samples should be examined to ensure that the product is still in compliance with the established specifications.
For more information, you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/US/en/life-science/quality-and-regulatory-management/quality-services.
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What concentration of Mitomycin C (Product M4287) for use with mesenchymal stem cells MSC?
1 answer-
The use of Mitomycin C (Product M4287) with mesenchymal stem cells (MSC) has not been investigated. This is an application that will need to be validated by the end user.
However, certain publications indicate the use of 1 mg/mL of mitomycin C stock solution in stromal cell culture.
This concentration may be suitable: https://www.sciencedirect.com/science/article/pii/S0014483520304590Helpful?
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How long can it be stored at 4 degrees when dissolved in water? How should I store it to keep it active for a longer period of time?
1 answer-
As mentioned in product information sheet, generally, solutions at pH 6–9 can be stored at 0–5 °C
for up to a week. For more information please see https://www.sigmaaldrich.com/product/sigma/m4287Helpful?
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
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What is the difference between Mitomycin C products M4287 and M0503?
1 answer-
M4287 has been cell culture tested; M0503 has not been cell culture tested.
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How should solutions of Mitomycin C, Product M4287, be stored?
1 answer-
Solutions of this product should not be stored frozen. Solutions can be stored at 2-8°C, and should be used within 1-2 weeks. Solutions are light sensitive, but the powder is not.
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At what concentration can Mitomycin C, Product M4287, be used with embryonic stem (ES) cells?
1 answer-
Treatment of the feeder layer with media containing 100 micrograms/mL mitomycin at 37°C for 30 minutes blocked replication of ES cells.
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What is the recommended concentration of Mitomycin C, Product M4287, should I use for eukaryote cell culture?
1 answer-
A concentration between 10-50 micrograms/mL is typically used.
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How should the product be dissolved?
1 answer-
Mitomycin C is soluble at 0.5 mg/mL in water, yielding a clear blue solution.
Helpful?
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