M1567

Malic Dehydrogenase from porcine heart

≥600 units/mg protein (biuret), ammonium sulfate suspension

• Sinónimos: L-Malate: NAD⁺ oxidoreductase, MDH, Malate Dehydrogenase
• Comisión internacional de enzimas: 1.1.1.37 (BRENDA, IUBMB)
• Número CE: 232-622-5
• Número MDL: MFCD00131567
• Código UNSPSC: 12352204
• NACRES: NA.54

Propiedades

• Formulario: ammonium sulfate suspension
• Nivel de calidad: 200
• actividad específica: ≥600 units/mg protein (biuret)
• actividad extraña: Glutamic-Oxalacetic Transaminase ≤0.01%; Glutamic-Pyruvic Transaminase ≤0.01%
• temp. de almacenamiento: 2-8°C

Descripción

Descripción general

Malic Dehydrogenase is a ubiquitous enzyme, which exists in two isoforms in eukaryotic cells.

Malic dehydrogenase exists as a dimer with each subunit containing an NAD-binding domain and a substrate-binding carboxy-terminal domain required for activity.

Malic dehydrogenase is a cytoplasmic isozyme and an important catalyst in the tricarboxylic acid cycle.

Aplicación

Malic Dehydrogenase from porcine heart has been used:

• in qualitative protein binding measurements
• to test internally calibrated electrochemical continuous enzyme assay (ICECEA) with model enzyme pair
• to investigate the effect of chaperone on the refolding of heat-denatured malate dehydrogenase

Malic dehydrogenase has been used in a study to assess the effect of an immunomodulator S2 complex on the enzymes of the parasites. It has also been used in a study to investigate the heterogeneity of lactic and malic dehydrogenase in cerebrospinal fluid.

Acciones bioquímicas o fisiológicas

Malic Dehydrogenase (MDH) plays an important role in the citric acid cycle in mitochondria. It catalyzes the interconversion of substrates malate and oxaloacetate with the simultaneous oxidation/reduction of NAD/NADH⁺. MDH present in the cytosol is involved in the shuttling of malate/aspartate.

Definición de unidad

One unit will convert 1.0 μmole of oxalacetate and β-NADH to L-malate and β-NAD per min at pH 7.5 at 25°C.

Forma física

Suspension in 2.8 M (NH₄)₂SO₄ solution, pH 6.0

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)