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L2542

Sigma-Aldrich

LB Broth (Miller)

Liquid microbial growth medium

Sinónimos:

Luria Broth Miller, Luria-Bertani broth, Miller

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About This Item

Código UNSPSC:
41106200
NACRES:
NA.85

grado

Molecular Biology
for molecular biology

esterilidad

sterile; 0.2 μm filtered

Formulario

liquid

técnicas

microbiological culture: suitable

pH

6.5-7.2

aplicaciones

microbiology

temp. de almacenamiento

room temp

idoneidad

nonselective for Escherichia coli
nonselective for coliforms

Descripción general

Miller’s LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates. It enables fast growth, improves growth yields for various bacterial species, and can retain the hydrophilic behavior of samples for longer periods.

Aplicación

LBBroth (Miller) is suitable for:

  • non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins.
  • selective cultivation when appropriate antibiotics are added.
  • the cultivation of E-coli cells for anti-bacterial assays.

Características y beneficios

Miller′s LB liquid provides:
  • Filter-sterilized, ready-to-use format
  • Convenient package size
  • Standard formulation

Componentes

10g/L Tryptone
10 g/L NaCl
5 g/L Yeast Extract

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Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

nwg

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificados de análisis (COA)

Lot/Batch Number
0000333808
0000333808
0000283405
SLCQ2569
0000259749

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Si necesita una versión concreta, puede buscar un certificado específico por el número de lote.

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Celine Abueva et al.
International journal of biological macromolecules, 182, 1713-1723 (2021-05-30)
The importance of developing more potent antimicrobials and robust infection prevention practices has been highlighted recently with the increase in reports of emerging bacterial resistance mechanisms and the development of antibiotic-resistant microbes. In this study, a quaternary ammonium chitosan derivative
Use of the Yeast Pichia pastoris as an Expression Host for Secretion of Enterocin L50, a Leaderless Two-Peptide (L50A and L50B) Bacteriocin from Enterococcus faecium L50
Antonio Basanta, Beatriz Gomez-Sala, et al.
Biotechnology, 76, 3314-3324 (2010)
Long-term retention of hydrophilic behavior of plasma treated polydimethylsiloxane (PDMS) surfaces stored under water and Luria-Bertani broth
Zhao LH, et al.
Sensors and actuators A, Physical, 181.0, 33-42 (2012)
Andrada-Ioana Damian-Buda et al.
Journal of functional biomaterials, 13(4) (2022-10-25)
Mesoporous bioactive glass nanoparticles (MBGNs) are widely recognized for their ability to bond to hard tissue, while the ions released from the BG structure enhance specific cellular pathways. In this study, the SiO2-P2O5-CaO-MgO-ZnO system was used to successfully synthesize MBGNs
Philippe M Fortune et al.
American journal of botany, 95(4), 454-464 (2008-04-01)
The origin of polyploid Bromus species of section Genea was investigated using molecular data. This group of annual species native from the Old-World is composed of three diploids, two tetraploids, one hexaploid, and one octoploid. Molecular cloning, sequencing, and phylogenetic
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Protocolos

Microbial Growth Protocols

General protocols for growth of competent cells in microbial medium.

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