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H9034

Sigma-Aldrich

Hepatitis C virus core antigen

~1 mg/mL, ≥95% (SDS-PAGE), recombinant, expressed in E. coli

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About This Item

MDL number:
MFCD02264926
UNSPSC Code:
12352202
NACRES:
NA.32

recombinant

expressed in E. coli

Quality Level

200

assay

≥95% (SDS-PAGE)

mol wt

22 kDa (plus 114 kDa galactosidse)

concentration

~1 mg/mL

UniProt accession no.

G9G988
L7S4V3

shipped in

dry ice

storage temp.

−20°C

Gene Information

hepatitis C virus ... HCVgp1(951475)
human ... HBVgp4(944568)

General description

Hepatitis C virus core antigen (HCV-cAg) is a well-preserved antigenic protein of the internal capsid.
Hepatitis C virus glycoprotein 1 (HCVgp1), also referred to as polyprotein, is crucial for the induction of double-membrane vesicles, sites of HCV RNA amplification. The amino terminal of polyprotein contains the structural proteins core, envelope protein 1 (E1) and E2. The proteolytic cleavage of polyprotein results in the formation of 10 mature viral proteins which are needed for viral replication and assembly.
amino acids 2-192 of HCV polyprotein

Specificity

Reacts strongly with human HCV positive serum.

Application

Hepatitis core antigen (HCA) has been used as an analyte in enzyme-linked immunosorbent assay (ELISA) to study the mechanism of bovine serum albumin (BSA) blocking in common ELISA protocols.

Biochem/physiol Actions

Hepatitis C virus core antigen (HCV-cAg) may be a less expensive way to diagnose active hepatitis C virus (HCV) infection in the hemodialysis (HD) population.
Positive control for HCV antibodies.

Other Notes

HCV core antigen [2-192]-Galactosidase-tagged at the N-terminus.

Physical form

Solution in 8 M Urea, 20 mM Tris-HCl, pH 8.0, 10 mM 2-mercaptoethanol.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Xue Zheng Wong et al.
BMC nephrology, 21(1), 480-480 (2020-11-15)
Hepatitis C virus (HCV) infects more than 71 million people worldwide and chronic HCV infection increases the risk of liver cirrhosis and failure. Haemodialysis (HD) is one of the renal replacement therapies with risk of HCV transmission. Anti-HCV antibodies are
Alejandra Pérez-García et al.
International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases, 89, 131-136 (2019-10-04)
New efficient strategies are needed for the assessment of active hepatitis C virus (HCV) infection. The aim of this study was to evaluate the ability of HCV core antigen (HCV-cAg) as a marker of active HCV infection in newly diagnosed
BSA blocking in enzyme-linked immunosorbent assays is a non-mandatory step: a perspective study on mechanism of BSA blocking in common ELISA protocols
Ahirwar R, et al.
Royal Society of Chemistry Advances, 5(121), 100077-100083 (2015)
Inés Romero-Brey et al.
mBio, 6(4), e00759-e00759 (2015-07-15)
Induction of membrane rearrangements in the cytoplasm of infected cells is a hallmark of positive-strand RNA viruses. These altered membranes serve as scaffolds for the assembly of viral replication factories (RFs). We have recently shown that hepatitis C virus (HCV)
Margaret A Scull et al.
PLoS pathogens, 11(11), e1005297-e1005297 (2015-11-21)
The hepatitis C virus (HCV) p7 protein is required for infectious virus production via its role in assembly and ion channel activity. Although NMR structures of p7 have been reported, the location of secondary structural elements and orientation of the

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