GE28-9321-71
HIS Spintrap™ Kit
Cytiva 28-9321-71, pack of 1 kit
About This Item
Productos recomendados
packaging
pack of 1 kit
manufacturer/tradename
Cytiva 28-9321-71
storage condition
(0.15% Kathon CG)
(20% Ethanol)
matrix
highly cross-linked 6% agarose
avg. part. size
34 μm
cleaning
2-14(Ni2+-stripped medium.)
working range
3-12(Ni2+-stripped medium.)
capacity
≥40 mg binding capacity (histidine-tagged protein/ml medium)(Protein binding capacity is protein-to-protein dependent.)
storage temp.
2-8°C
Categorías relacionadas
General description
Features and Benefits
- For simple, small-scale purification of histidine-tagged* proteins and rapid expression screening.
- Ni Sepharose High Performance in prepacked spin columns.
- Yields up to 750 µg pure protein per column.
- Allows direct purification of both clarified and unclarified cell lysates.
Components
1 pack - GE28-4013-53 - His Spin Trap™
1 pack - GE11-0034-00 - His Buffer Kit
Storage and Stability
Analysis Note
Legal Information
Los componentes del kit también están disponibles por separado
- GE11-0034-00His Buffer Kit, Cytiva 11-0034-00SDS
- GE28-4013-53His Spintrap™, Cytiva 28-4013-53SDS
signalword
Danger
hcodes
Storage Class
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Artículos
How to optimize purification of histidine-tagged proteins using Cytiva products.
This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from Cytiva.
This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from Cytiva.
This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from Cytiva.
Protocolos
This page describes a small-scale purification (minipreps) of histidine-tagged recombinant proteins using His SpinTrap and His SpinTrap Kit from Cytiva.
Ni Sepharose High Performance consists of highly cross-linked 6% agarose beads (34 µm) to which a chelating group has been immobilized and subsequently charged with Ni2+ ions.
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