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Merck
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Key Documents

GE28-4064-14

Sigma-Aldrich

Tricorn 10/50 Column

Sinónimos:

Tricorn column

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About This Item

UNSPSC Code:
41121800
NACRES:
SB.52

technique(s)

LPLC: suitable

General description

Tricorn 5 and 10 Coarse Filter Kit is used for large volume/repeated loading of clarified sample to reduce the risk of clogging in combination with capture media. Both filter and coarse filter kits include top and bottom adapters, bottom filters, and EPDM O-rings, five of each.
Tricorn Packing Equipment includes the Packing Equipment 5/50 (5-mm connector and 50-mm glass tube) or 10/100 (10-mm connector and 100-mm glass tube), EPDM O-rings and bottom unit, cap, and stop plug.
Tricorn columns include a glass tube, adapter unit, end cap, filter kit, two stop plugs, two fingertight connectors, two M6 connectors, adapter lock, and filter holder.

Features and Benefits

Broad range of column dimensions suitable for many different purification needs.
Glass tubes coated with plastic film prevent harm if the column is dropped or operated outside its specification.
Locking ring prevents accidental compression of media bed.
Separate packing connector allows simple column packing for a broad range of media.
The modern integrated, snap-on design results in a column that is easy to assembly, operate, and maintain.
Tricorn empty columns are designed for high performance separations on small and medium scale. Pressure stability up to 100 bar and durable column material ensure reliable and reproducible operation as well as compatibility with the majority of the commercial lab media.

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Artículos

This page describes efficient column packing and preparation for affinity chromatography of antibodies.

This page shows how to convert between flow velocity and volumetric flow rate in affinity chromatography of antibodies.

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.

Protocolos

This page shows how to purify histidine-tagged proteins using TALON Superflow from Cytiva.

This page shows how to separate IgG antibodies by affinity chromatography using Protein G Sepharose 4 Fast Flow from Cytiva.

This page describes efficient column packing and preparation for affinity chromatography of GST-tagged proteins using Cytiva products.

This page shows how to purify histidine-tagged proteins secreted into eukaryotic cell culture supernatants using Ni Sepharose Excel from Cytiva.

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