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Merck

G4296

Sigma-Aldrich

GSK3β, active, His tagged human

PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

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About This Item

Código UNSPSC:
12352200
NACRES:
NA.32

recombinante

expressed in baculovirus infected Sf9 cells

Nivel de calidad

Línea del producto

PRECISIO® Kinase

Ensayo

≥70% (SDS-PAGE)

Formulario

buffered aqueous glycerol solution

actividad específica

143-193 nmol/min·mg

mol peso

~48 kDa

Nº de acceso UniProt

Condiciones de envío

dry ice

temp. de almacenamiento

−70°C

Información sobre el gen

human ... GSK3B(2932)

Descripción general

Research area: Cellsignalling. GSK3B (glycogen synthase kinase 3β) is one of the two isoforms of GSK3 enzyme, both of which are encoded by different genes. This protein is predominantly cytoplasmic, but is also found to be localized to nucleus and mitochondria. It is a protein-serine kinase. This protein contains a protein kinase domain in its core, and is composed of 482 amino acids with a molecular weight of 46,712Da. It shows a wide range of tissue expression with the highest expression in brain.

Aplicación

GSK3β, active, His tagged human has been used as an incubating medium during in vitro hyperphosphorylation of tau protein.

Acciones bioquímicas o fisiológicas

GSK3β is a serine-threonine protein kinase that was originally identified as the kinase that phosphorylates and inhibits glycogen synthase.GSK3β is ubiquitously present in human tissues and implicated in the regulation of several physiological processes, including the control of glycogen and protein synthesis by insulin and modulation of the transcription factors AP-1 and CREB. Transient transfection of human GSK3β into Chinese hamster ovary cells stably transfected with individual human tau isoforms leads to hyperphosphorylation of tau at all the sites investigated with phosphorylation-dependent anti-tau antibodies.
Glycogen Synthase Kinase 3β (GSK3β) is responsible for rapid cyclin D1 turnover by phosphorylating it on Thr-286. It functions as a negative regulator of Wnt signaling during embryonic development and cell proliferation in adult tissues. This protein phosphorylates and regulates multiple proteins, such as glycogen synthase, insulin receptor substrate-1, axin and APC (adenomatous polyposis coli). This protein is also shown to interact with multiple proteins which are linked with Alzheimer′s disease (AD).

Forma física

Supplied in 50mM NaPhosphate pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1mM PMSF, 0.2 mM DTT, 25% glycerol.

Información legal

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictogramas

Health hazardExclamation mark

Palabra de señalización

Danger

Frases de peligro

Clasificaciones de peligro

Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2

Código de clase de almacenamiento

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter


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Lannah S Abasi et al.
Communications biology, 7(1), 251-251 (2024-03-02)
Tau is a microtubule-associated protein often found in neurofibrillary tangles (NFTs) in the brains of patients with Alzheimer's disease. Beyond this context, mounting evidence suggests that tau localizes into the nucleus, where it may play a role in DNA protection
B R Sperber et al.
Neuroscience letters, 197(2), 149-153 (1995-09-08)
Hyperphosphorylated tau protein is the major constituent of the paired helical filament (PHF), the major fibrous component of the neurofibrillary lesions of Alzheimer's disease (AD). Hyperphosphorylation of tau is believed to be the critical event that leads to filament assembly.
Glycogen synthase kinase 3 in Wnt signaling pathway and cancer
Nydia Tejeda-Mu?oz
IUBMB Life (2015)
Richard S Jope et al.
Trends in biochemical sciences, 29(2), 95-102 (2004-04-23)
Glycogen synthase kinase-3 (GSK3) is now recognized as a key component of a surprisingly large number of cellular processes and diseases. Several mechanisms play a part in controlling the actions of GSK3, including phosphorylation, protein complex formation, and subcellular distribution.
T Hennet
Cellular and molecular life sciences : CMLS, 59(7), 1081-1095 (2002-09-12)
Galactose is transferred via several linkages to acceptor structures by galactosyltransferase enzymes. In prokaryotes, galactose is mainly found on lipopolysaccharides and capsular polysaccharides. In eukaryotes, galactosyltransferases, which are localized in the Golgi apparatus, are involved in the formation of several

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