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Merck
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F6257

Sigma-Aldrich

Anti-Mouse IgG (whole molecule)–FITC antibody produced in sheep

affinity isolated antibody, buffered aqueous solution

Sinónimos:

Sheep Anti-Mouse IgG (whole molecule)–Fluorescein isothiocyanate

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About This Item

MDL number:
MFCD00162642
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

sheep

conjugate

FITC conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

technique(s)

direct immunofluorescence: 1:32

storage temp.

2-8°C

target post-translational modification

unmodified

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General description

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections
Anti-Mouse IgG (whole molecule)-FITC antibody is specific for all mouse IgG subclasses. Affinity isolated antigen specific antibody is obtained from sheep anti-mouse IgG antiserum by immunospecific purification. The antibody preparation is then conjugated to Sigma Fluorescein Isothiocyanate (FITC), Isomer I.

Immunogen

Purified mouse IgG

Application

Anti-Mouse IgG (whole molecule)-FITC antibody may be used for immunofluorescent labeling of mouse spleen cells at a working antibody dilution of 1:32. For immunofluorescence of human ovarian adenocarcinoma cells stained with anti-integrin primary antibodies a working dilution of 1:50 was used. Immunolocalization of human spermatids was done using antibody dilution of 1:100. This antibody was also used for immunofluorescent tagging of leaf cells of Arabidopsis.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

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Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Fiona Ingrao et al.
Vaccines, 9(7) (2021-08-07)
Recombinant Newcastle disease viruses (rNDV) have been used as bivalent vectors for vaccination against multiple economically important avian pathogens. NDV-vectored vaccines expressing the immunogenic H5 hemagglutinin (rNDV-H5) are considered attractive candidates to protect poultry from both highly pathogenic avian influenza
Lénaïg Louboutin et al.
Journal of fish diseases, 45(5), 707-716 (2022-02-17)
Gilthead seabream represents a species of importance in Mediterranean aquaculture. The larval stage is particularly sensitive and frequently impacted in suboptimal environmental or sanitary conditions. In the present study, investigations were carried out in a seabream hatchery following an unusual
Catarina Rapôso et al.
PloS one, 9(9), e107292-e107292 (2014-09-12)
Severe accidents caused by the "armed" spider Phoneutria nigriventer cause neurotoxic manifestations in victims. In experiments with rats, P. nigriventer venom (PNV) temporarily disrupts the properties of the BBB by affecting both the transcellular and the paracellular route. However, it
A O Queiroz et al.
Memorias do Instituto Oswaldo Cruz, 96(7), 965-972 (2001-10-31)
"Mal de Cadeiras", an enzootic disease caused by Trypanosoma evansi, is one of the most important trypanosomiases in the Brazilian Pantanal region. The disease affects mainly horses, which are widely used in extensive cattle production, an activity of greatest economical
U I Ezeh et al.
Human reproduction (Oxford, England), 13(11), 3061-3065 (1998-12-16)
Limiting testicular biopsy for intracytoplasmic sperm injection (ICSI) to those with a high chance of having testicular spermatozoa has not been possible because of the poor predictive value of current clinical and laboratory methods. In order to predict testicular pathology
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