E3024

Earle′s Balanced Salts

With sodium bicarbonate, without phenol red, liquid, sterile-filtered, suitable for cell culture

• Sinónimos: EBSS
• UNSPSC Code: 12352207
• NACRES: NA.75
• MDL number: MFCD00217343

Propiedades

• Quality Segment: 400
• sterility: sterile-filtered
• form: liquid
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• components: phenol red: no; NaHCO₃: 2.2 g/L; glucose: 1.0 g/L (Dextro)
• shipped in: ambient
• storage temp.: room temp

Descripción

General description

Earle′s balanced salts solution (EBSS) is a saline solution with physiological pH that stimulates autophagy. It augments Bacillus Calmette-Guérin (BCG)-induced dendritic cells (DCs) by upregulating cluster of differentiation (CD86), human leukocyte antigen-DR isotype (HLA-DR), and interleukin (IL-6).

Application

Earle′s balanced salts has been used to incubate filter-grown monolayers of Caco-2 cells to induce starvation, along with HEPES buffer to detect NADPH oxidase activity in trypsinized cells. It has also been used to treat CEM RES cells and perform amino acid depletion experiment.

Other Notes

Phenol red has been shown to interfere with the growth of some cells at cloning densities. Use this medium when working with stem cells or when growing cells at low densities.

Información de seguridad

• Clase de almacenamiento: 12 - Non Combustible Liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable