This product contains two substrate tablets per tablet set; there is one DAB/cobalt tablet and one urea/hydrogen peroxide tablet. Both tablets must be used in the assay.
D0426
DAB with Metal Enhancer
SIGMAFAST™, peroxidase substrate, tablet
Sinónimos:
3,3′-Diaminobenzidine Peroxidase Substrate, DAB Peroxidase Substrate
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About This Item
UNSPSC Code:
12352204
NACRES:
NA.83
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Nombre del producto
SIGMAFAST™ DAB with Metal Enhancer, tablet
form
tablet
Quality Level
solubility
water: 1 tablet/5 mL
Storage temp.
−20°C
General description
SIGMAFAST™ DAB with Metal Enhancer Tablets require no additional buffers or steps to prepare an active substrate solution for detection of peroxidase activity. Commom use is in immunohistology as a precipitating substrate.
Application
SIGMAFAST™ DAB with Metal Enhancer has been used in the visualization of lung tissue sections using immunohistochemistry,[1] dorsal root ganglion (DRG) neurons[2] and immunohistochemical staining for collagen in anterior cruciate ligament (ACL) cells.[3]
The DAB reaction is enhanced by the addition of CoCl2 and produces a more intense stain which is resistant to alcohol.
Reconstitution
Each tablet set dissolved in 5 mL deionized water yields a ready-to-use buffered solution containing DAB with a metal enhancer.
Legal Information
SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC
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Danger
Hazard Classifications
Aquatic Chronic 2 - Carc. 1B - Eye Dam. 1 - Muta. 2 - Repr. 1B - Resp. Sens. 1 - Skin Sens. 1
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
flash_point_f
Not applicable
flash_point_c
Not applicable
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Artículos
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
Protocolos
Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.
Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.
Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.
Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.
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What is a tablet set for Product D0426, SIGMAFAST™ DAB with Metal Enhancer?
1 answer-
Helpful?
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
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How can I block endogenous peroxidase activity when using Product D0426, SIGMAFAST™ DAB with Metal Enhancer, in immunohistochemistry?
1 answer-
Endogenous peroxidase activity may be blocked by flooding the slide with a solution of 4 parts methanol and 1 part 3% hydrogen peroxide.
Helpful?
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What is the metal enhancer in Product D0426, SIGMAFAST™ DAB with Metal Enhancer, and what is the purpose of it?
1 answer-
The metal enhancer is cobalt chloride (CoCl2). Cobalt chloride produces a more intense stain which enhances the sensitivity of the assay and allows for visualization of the staining when a standard DAB chromogen would fail to demonstrate the staining.
Helpful?
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