CS1090
HMG-CoA Reductase Assay Kit
sufficient for 30 assays (1 mL), sufficient for 100 assays (200 μL)
Sinónimos:
HMGR Assay Kit
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About This Item
Código UNSPSC:
12161503
NACRES:
NA.28
Productos recomendados
uso
sufficient for 100 assays (200 μL)
sufficient for 30 assays (1 mL)
Nivel de calidad
Condiciones de envío
dry ice
temp. de almacenamiento
−70°C
Información sobre el gen
human ... HMGCR(3156)
Categorías relacionadas
Descripción general
The HMG-CoA Reductase Assay Kit is an important tool for the basic research of cholesterol and other related metabolic pathways.
Aplicación
The HMGR activity kit is designed for the detection of HMG-CoA reductase activity. A major function of the kit is to screen for various inhibitors and activators of the purified catalytic subunit of the enzyme, HMG-CoA reductase, which may play a crucial role in therapeutics. The assay is based on the spectrophotometric measurement of the decrease in absorbance at 340 nm, which represents the oxidation of NADPH by the catalytic subunit of HMGR in the presence of the substrate HMG-CoA.
Acciones bioquímicas o fisiológicas
HMGR (3-hydroxy-3-methylglutaryl-CoA reductase) is a transmembrane glycoprotein located on the endoplasmic reticulum. This enzyme catalyzes the four-electron reduction of HMG-CoA to coenzyme A (CoA) and mevalonate, which is the rate-limiting step in sterol biosynthesis. The activity of HMGR is controlled through synthesis, degradation, and phosphorylation in order to maintain the concentration of mevalonate derived products. In addition to the physiological regulation of HMGR, the human enzyme has been targeted successfully by drugs in the clinical treatment of high serum cholesterol levels. Controlling serum cholesterol levels has an important therapeutic role since hypercholesterolemia often leads to the development of atherosclerosis and subsequently to cardiovascular pathologies, which might result in myocardial infarction and stroke. It has been suggested that a disturbance of cholesterol homeostasis contributes to the development of a chronic inflammatory state.
Los componentes del kit también están disponibles por separado
Referencia del producto
Descripción
SDS
- N6505β-Nicotinamide adenine dinucleotide 2′-phosphate reduced tetrasodium salt hydrate, ≥95% (HPLC) 25 mgSDS
Código de clase de almacenamiento
11 - Combustible Solids
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Los clientes también vieron
G A Holdgate et al.
Biochemical Society transactions, 31(Pt 3), 528-531 (2003-05-30)
The statins are inhibitors of 3-hydroxy-3-methylglutaryl CoA (HMG-CoA) reductase (HMG-CoAR), and are utilized to decrease levels of atherogenic lipoproteins in patients with, or who are at high risk of, cardiovascular disease. This study describes the inhibition of a recombinant, catalytic
R Kleemann et al.
Current drug targets. Cardiovascular & haematological disorders, 5(6), 441-453 (2006-03-01)
Besides classical risk factors such as hypercholesterolemia and hypertension, chronic subacute inflammation has recently been recognized as an important force driving the development of atherosclerosis, the most common underlying cause of myocardial infarction and stroke. There is compelling evidence that
Matteo Mozzicafreddo et al.
Journal of lipid research, 51(8), 2460-2463 (2010-04-27)
Radioisotope-based and mass spectrometry coupled to chromatographic techniques are the conventional methods for monitoring HMG-CoA reductase (HMGR) activity. Irrespective of offering adequate sensitivity, these methods are often cumbersome and time-consuming, requiring the handling of radiolabeled chemicals or elaborate ad-hoc derivatizing
A J Koning et al.
Molecular biology of the cell, 7(5), 769-789 (1996-05-01)
In all eucaryotic cell types analyzed, proliferations of the endoplasmic reticulum (ER) can be induced by increasing the levels of certain integral ER proteins. One of the best characterized of these proteins is HMG-CoA reductase, which catalyzes the rate-limiting step
Rosana Aparecida Manólio Soares et al.
International journal of molecular sciences, 16(2), 4150-4160 (2015-02-19)
The objective of this study was to identify the major peptides generated by the in vitro hydrolysis of Amaranthus cruentus protein and to verify the effect of these peptides on the activity of 3-hydroxy-3-methyl-glutaryl-CoA reductase (HMG-CoA reductase), a key enzyme
Artículos
3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) is a transmembrane glycoprotein, located on the endoplasmic reticulum.
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