CS0410

Glutathione-S-Transferase (GST) Assay Kit

sufficient for 500 multiwell tests

• Sinónimos: GST Enzyme Activity Kit
• UNSPSC Code: 12161503
• EC Number: 202-551-4
• NACRES: NA.84

Propiedades

• usage: sufficient for 500 multiwell tests
• Quality Segment: 200
• shipped in: dry ice
• storage temp.: −20°C
• Gene Information: human ... GSTA4(2941), GSTM1(2944)

Descripción

General description

The Glutathione S-Transferase (GST) Assay Kit is designed to measure total GSTactivity. It can be used to measure GST activity in cell and bacterial lysates, tissue homogenates, and in plasma
and erythrocyte lysates

Application

The Glutathione S-Transferase Assay kit utilizes 1-Chloro-2,4-dinitrobenzene (CDNB), which is suitable for the broadest range of GST isozymes. Upon conjugation of the thiol group of glutathione to the CDNB substrate, there is an increase in the absorbance at 340 nm.

Biochem/physiol Actions

Glutathione S-transferases (GSTs) are a group of enzymes important in the detoxification of many xenobiotics in mammals. The enzymes protect cells against toxicants by conjugating the thiol group of the glutathione to electrophilic xenobiotics, and thereby defend cells against the mutagenic, carcinogenic, and toxic effects of the compounds. GST activity is present in plants, insects, yeast, bacteria, and most mammalian tissues especially in the liver, which plays a key role in detoxification.

Solo componentes del kit

• Dulbecco's Phosphate Buffered Saline 100 mL
• L-Glutathione Reduced 1 g
• GST (Control) .1 mL
• Sample Buffer 5 mL
• Substrate (CDNB) 1.2 mL

Información de seguridad

• signalword: Danger
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• pictograms: GHS02,GHS07,GHS09
• hcodes: H225,H317,H319,H410
• pcodes: P210 - P233 - P273 - P280 - P303 + P361 + P353 - P305 + P351 + P338
• Hazard Classifications: Aquatic Acute 1 - Aquatic Chronic 2 - Eye Irrit. 2 - Flam. Liq. 2 - Skin Sens. 1
• Clase de almacenamiento: 3 - Flammable liquids