RT4

91091914, human bladder, Epithelial

• Sinónimos: RT-4 Cells, RT4PCells
• UNSPSC Code: 41106514

Propiedades

• product name: RT4, 91091914
• biological source: human bladder
• growth mode: Adherent
• karyotype: Not specified
• morphology: Epithelial
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Descripción

Cell Line Origin

Human Caucasian bladder transitional-cell carcinoma

Cell Line Description

Derived from explants of a recurring papillary tumour of the bladder.

Application

RT4 cell line has been used to study the effects of uropathogenic Escherichia coli on neutrophil adhesion via neutrophil adhesion assay.

Tumourigenicity studies

DNA Profile

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 8
D16S539: 9
D5S818: 11,12
D7S820: 9,12
THO1: 9,9.3
TPOX: 8,11
vWA: 14,17

Culture Medium

McCoy′s 5a + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-5x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C.

Other Notes

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