TM4

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 88111401, mouse testis, Not specified

• Código UNSPSC: 41106514

Propiedades

• origen biológico: mouse testis
• envase: tube of 5 μg 88111401-DNA-5UG; pkg of vial of cells 88111401-1VL
• modo de crecimiento: Adherent
• cariotipo: Not specified
• morfología: Not specified
• productos: Transferrin, H-Y Ag, novel retinol-binding protein
• receptores: Androgen, oestrogen and progesterone receptors
• técnicas: cell culture | mammalian: suitable
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Mouse BALB/c testis sertoli cell

Descripción línea celular

From primary culture of sertoli cell enriched preparations from normal testes of a 11-13 day old BALB/c nu/+ mice. Cells respond to FSH but not LH; Plasminogen activator secretion is stimulated by FSH and also retinoic acid. Note: only distributed frozen.

Aplicación

Hormone studies

Medio de cultivo

Ham′s F12 + DMEM (1:1) + 2mM Glutamine + 5% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:20 to 1:200 i.e. seeding at 1x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Cells resuscitated from frozen stock should be split 1:3 at the first passage and thereafter as above.

Otras notas

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