LS180

87021202, human colon, Epithelial-like

• Código UNSPSC: 41106514

Propiedades

• Nombre del producto: LS180, 87021202
• origen biológico: human colon
• modo de crecimiento: Adherent
• cariotipo: Modal no. 45 (2n=46)
• morfología: Epithelial-like
• productos: Carcinoembryonic antigen (CEA), Mucin
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Human Caucasian colon adenocarcinoma

Descripción línea celular

Derived from a 58 year old female Caucasian with Dukes type B adenocarcinoma of the colon. The cells secrete CEA and mucin and are tumorigenic in nude mice. LS174T (Sigma Catalogue number. 87060401) was derived from the same patient. The cells have not been exposed to trypsin.

Aplicación

Tumourigenicity studies

Medio de cultivo

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-5x10,000 cells/cm² by scraping; 5% CO₂; 37°C.

Otras notas

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