Mero-25

9100102, human lung, Mixed epithelial and spindle shaped

• Código UNSPSC: 41106514

Propiedades

• Nombre del producto: Mero-25, 09100102
• origen biológico: human lung
• modo de crecimiento: Adherent
• cariotipo: Number of chromosomes 127-140
• morfología: Mixed epithelial and spindle shaped
• productos: Cytokeratins 7, 8, 18 and 19. Epithelial membrane antigen (EMA)
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

A human mesothelioma cell line derived from a malignant mesothelioma. Asbestos exposure

Descripción línea celular

Mero-25 was derived from autopsy tumour material from a male patient aged >60 with a history of asbestos exposure

Aplicación

Investigation of the properties of malignant mesothelioma cells and the development of new diagnostic methods

Perfil del ADN

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 12
D16S539: 11,12
D5S818: 11,12
D7S820: 9
THO1: 8
TPOX: 9
vWA: 16,17

Medio de cultivo

Hams F10 + 15% FCS + 2mM Glutamine

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Population doubling approx 48 hours

Otras notas

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