WERI

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 6070602, human eye, Grape like clusters of round cells

• Código UNSPSC: 41106514

Propiedades

• origen biológico: human eye
• envase: tube of 5 μg 06070602-DNA-5UG; pkg of vial of cells 06070602-1VL
• modo de crecimiento: Semi-adherent
• cariotipo: Not specified
• morfología: Grape like clusters of round cells
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Human Retinoblastoma

Descripción línea celular

Human retinoblastoma cell line derived from a female Caucasian. The cells are semi-adherent, some neuronal differentiation can occur when subculturing. Tumourigenic in rabbits.

Medio de cultivo

Iscove′s Modified DMEM + 2mM Glutamine + 15% Foetal Bovine Serum (FBS) + 10ug/ml Insulin + 55uM 2-Mercaptoethanol

Rutina de subcultivo

Split subconfluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-3 x100,000 cells/ml; 5% CO₂; 37°C. Saturation density: 2 x 1000,000 cells/ml. Cells are semi-adherent. Scrape cells from surface; do not use trypsin.

Otras notas

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