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CASP3F

Sigma-Aldrich

Caspase 3 Assay Kit, Fluorimetric

Sinónimos:

Caspase-3 Activity Fluorometric Kit

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About This Item

EC Number:
247-454-8
UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 1,000 multiwell tests
 sufficient for 100 standard tests (2 ml)

Quality Level

200

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... CASP3(836)

General description

The Caspase 3 Fluorometric Assay Kit is based on the hydrolysis of acetyl Asp-Glu-Val-Asp 7-amido-4-methylcoumarin (Ac-DEVD-AMC) by caspase 3, resulting in the release of the fluorescent 7-amino-4-methylcoumarin (AMC). The excitation and emission wavelengths of AMC are 360 nm and 460 nm respectively. The concentration of the AMC released can be calculated from a calibration curve prepared with AMC standards (AMC standard included with the kit).

Application

The Caspase 3 Fluorimetric Assay Kit is designed for the detection and measurement of caspase 3 activity in cell extracts or purified preparations of caspase 3.

Unit Definition

One unit of caspase 3 will cleave 1.0 μmol of Ac-DEVD per min at pH 7.4 at 25 °C.

Solo componentes del kit

Referencia del producto
Descripción
  • Water (17-18 megaOhm) 125 mL

Los componentes del kit también están disponibles por separado

Referencia del producto
Descripción
SDS
  • A1086Ac-DEVD-AMC substrate 2.5 mgSDS
  • A0835Ac-DEVD-CHO inhibitor .5 mgSDS
  • A98917-Amino-4-methylcoumarin standard 1 mgSDS

related product

Referencia del producto
Descripción
Precios

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H319

Hazard Classifications

Eye Irrit. 2

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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