C8058

Chondroitinase B from Flavobacterium heparinum

lyophilized powder (with BSA as stabilizer)

• Sinónimos: Chondroitin sulfate B lyase
• Número CAS: 52227-83-5
• UNSPSC Code: 12352204
• NACRES: NA.54
• MDL number: MFCD00166444
• Biological source: bacterial (Flavobacterium heparinum)

Propiedades

• biological source: bacterial (Flavobacterium heparinum)
• Quality Segment: 200
• conjugate: (Glucosaminoglycan)
• form: lyophilized powder (with BSA as stabilizer)
• shipped in: dry ice
• storage temp.: −20°C

Descripción

Application

The enzyme from Sigma has been used for the analysis of the frequency of iduronic acid in dermatan sulfate dodecasaccharide. It has also been used to digest dermatan sufate (DS) in melanoma cells. This digestion resulted in decreased proliferation and invasiveness of tumor cells, thereby suggesting a role for DS in metastasis.

Chondroitinase B from Flavobacterium heparinum has been used in a study to assess the structural characterization and antithrombin activity of dermatan sulfate. Chondroitinase B from Flavobacterium heparinum has also been used in a study to investigate the chondroitin lyase action pattern via liquid chromatography–mass spectrometry.

Biochem/physiol Actions

Chondroitinase B degrades only chondroitin sulphate B producing oligo- and tetra-saccharides, and an unsaturated 4-sulphated disaccharide. It has an optimum temperature of 20 °C and an optimum pH of 8.0. The enzyme activity is inhibited by 50% using 0.1 M NaCI. Co²⁺, Fe³⁺ and Ba²⁺ also inhibit its activity.

Other Notes

One unit will form 0.1 μmole of unsaturated uronic acid per hr at pH 7.5 at 25°C using chondroitin sulfate B as substrate.

Información de seguridad

• Pictogramas: GHS07
• Palabra de advertencia: Warning
• Códigos de peligro: H319
• Indicaciones de precaución: P264 - P280 - P305 + P351 + P338 - P337 + P313
• Hazard Classifications: Eye Irrit. 2
• Clase de almacenamiento: 13 - Non Combustible Solids
• WGK: WGK 3
• Punto de inflamación (°F): Not applicable
• Punto de inflamación (°C): Not applicable
• EPI (equipos de protección individual): Eyeshields, Gloves, type N95 (US)