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C3411

Sigma-Aldrich

2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein

≥90% (HPLC)

Sinónimos:

BCECF

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About This Item

Fórmula empírica (notación de Hill):
C27H20O11
Número de CAS:
85138-49-4
Peso molecular:
520.44
Beilstein/REAXYS Number:
8375770
MDL number:
MFCD00036970
UNSPSC Code:
12352200
PubChem Substance ID:
24892612
NACRES:
NA.32

Quality Level

100

assay

≥90% (HPLC)

form

powder

storage temp.

−20°C

SMILES string

OC(=O)CCc1cc2c(OC3=CC(=O)C(CCC(O)=O)=CC3=C2c4cc(ccc4C(O)=O)C(O)=O)cc1O

InChI

1S/C27H20O11/c28-19-10-21-17(7-12(19)2-5-23(30)31)25(16-9-14(26(34)35)1-4-15(16)27(36)37)18-8-13(3-6-24(32)33)20(29)11-22(18)38-21/h1,4,7-11,28H,2-3,5-6H2,(H,30,31)(H,32,33)(H,34,35)(H,36,37)

InChI key

DKROGDOAOXDINY-UHFFFAOYSA-N

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Application

2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein has been used in a fluorescence-based screening assay, to determine the pH.

Biochem/physiol Actions

2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF) is useful to study multidrug resistance-associated protein (MRP) transporter action within the cell. BCECF is known to interfere with drug efflux interactions.
Fluorescent indicator for measurement of cytoplasmic pH in lymphocytes.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Adelaide S M Dennis et al.
Scientific reports, 8(1), 8795-8795 (2018-06-13)
Four hundred structurally diverse drug-like compounds comprising the Medicines for Malaria Venture's 'Pathogen Box' were screened for their effect on a range of physiological parameters in asexual blood-stage malaria (Plasmodium falciparum) parasites. Eleven of these compounds were found to perturb
Alexander A Tokmakov et al.
Biochemical and biophysical research communications, 517(1), 140-145 (2019-07-20)
Mitogen-activated protein kinases (MAPKs) are involved in the regulation of various cellular processes, including cell survival and apoptosis. Here, we report that Xenopus p42 MAPK becomes phosphorylated in apoptotic eggs, however this modification does not activate the enzyme. Using phosphorylation
High-throughput workflow for monitoring and mining bioprocess data: Application to infer E. coli physiological response to perturbations.
Heux S, et al.
Applied and Environmental Microbiology, AEM-05838 (2011)
Patrick Hofmann et al.
Cardiovascular research, 115(1), 230-242 (2018-08-15)
Long non-coding RNAs (lncRNAs) have been shown to regulate numerous processes in the human genome, but the function of these transcripts in vascular aging is largely unknown. We aim to characterize the expression of lncRNAs in endothelial aging and analyse
Chang-Yin Lee et al.
Oncology reports, 37(1), 399-407 (2016-11-15)
Panax notoginseng (P. notoginseng) and its components are used as traditional Chinese medicine for cardiovascular disease, although studies concerning the anti-metastatic properties of these compounds are limited. The goal of this study was to investigate the effects of notoginsenoside R1 (NGR1), an important
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