While long term stability studies have not been conducted, stock solutions of this material are expected to be stable for at least 6 months, when stored at 2-8°C. Note that product C0663, Acetylcholinesterase from Erythrocytes, a solution prepared in HEPES buffer pH 8.0 w/0.1% TRITON® X-100, is assigned a retest date of approximately 2 years from the date of Quality Release. This item is stored at 2-8°C.
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Acerca de este artículo
type
Type VI-S
Quality Segment
form
lyophilized powder
specific activity
200-1,000 units/mg protein
mol wt
280 kDa
composition
Protein, ≥45% biuret
solubility
20 mM Tris HCl buffer, pH 7.5: soluble 1.0 mg/mL, clear(lit.)
application(s)
diagnostic assay manufacturing
storage temp.
−20°C
General description
Punto isoeléctrico: 5,5
Coeficiente de extinción: E1% = 18,0 (280 nm)
La acetilcolinesterasa de Electrophorus electricus es un tetrámero compuesto de 4 subunidades iguales de 70 kDa cada una. Cada subunidad contiene un sitio activo. La enzima es una glucoproteína que contiene hexosaminas.
Application
Biochem/physiol Actions
Physical form
Preparation Note
Analysis Note
Other Notes
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Este artículo | |||
|---|---|---|---|
| specific activity 200-1,000 units/mg protein | specific activity ≥1,000 units/mg protein | specific activity ≥900 units/mg protein | specific activity ≥1,000 units/mg protein (BCA) |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| application(s) diagnostic assay manufacturing | application(s) - | application(s) diagnostic assay manufacturing | application(s) - |
| mol wt 280 kDa | mol wt 280 kDa | mol wt tetramer 440 kDa | mol wt 64.6 kDa |
| solubility 20 mM Tris HCl buffer, pH 7.5: soluble 1.0 mg/mL, clear(lit.) | solubility Tris buffer: 1 mg/mL (0.02 M Tris buffer, pH 7.5) | solubility H2O: soluble 10 mg/mL | solubility - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
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Clase de almacenamiento
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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How can I store this enzyme after dissolving it in Tris Buffer for long term?
1 answer-
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Could you please provide the Uniprot accession number for this product? Also, in general, it would be very helpful if this information were included for any items in your catalog that are proteins. Thanks!
1 answer-
UniProt number O42275 is for Protein Acetylcholinesterase, Organism Electrophorus electricus (Electric eel) (Gymnotus electricus).
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The bottle mentions the concentration as 200-1000 U/mg. What exact concentration should we consider for calculation purposes?
1 answer-
The exact concentration is listed on the lot specific Certificate of Analysis. Please see the sample certificate of analysis: https://www.sigmaaldrich.com/certificates/COFA/C3/C3389/C3389-BULK________SLCJ8803__.pdf
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