C3062
Anti-Cholera Toxin antibody produced in rabbit
whole antiserum
Sinónimos:
Cholera Toxin Antibody
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About This Item
Productos recomendados
origen biológico
rabbit
conjugado
unconjugated
forma del anticuerpo
whole antiserum
tipo de anticuerpo
primary antibodies
clon
polyclonal
contiene
15 mM sodium azide
técnicas
Ouchterlony double diffusion: 1:16
dot blot: 1:20,000
indirect ELISA: 1:8,000
Condiciones de envío
dry ice
temp. de almacenamiento
−20°C
modificación del objetivo postraduccional
unmodified
Descripción general
Cholera toxin, the pathogenic agent of cholera, is made of two subunits, A (27 kDa) and B (12 kDa) assembled with the stoichiometry AB5. The B-subunit binds to specific receptors, the monosialogangliosides GM1, located in the membrane of intestinal epithelial cells. The A1 fragment of the A-subunit is translocated through the membrane of the host cell, where it catalyses the ADP-ribosylation of the Gsa regulatory component of the adenylate cyclase complex. The resulting increased level of cyclic AMP promotes a wide variety of actions, including the secretion of chloride ions in the case of intestinal epithelial cells.
The antiserum reacts versus Cholera toxin, but shows no reaction versus Staphylococcal enterotoxin A, Staphylococcal enterotoxin B and Pseudomonas exotoxin A (protein concentration: 50-500 ng/dot). The product has not been tested for neutralization potency against active Cholera toxin.
Inmunógeno
toxin from Vibrio cholerae
Aplicación
Anti-Cholera Toxin antibody produced in rabbit has been used in:
- western blotting
- quantitative ganglioside-dependent enzyme-linked immunoassay (ELISA)
- double immunodiffusion
Acciones bioquímicas o fisiológicas
Cholera toxin (CT), a multifunctional protein plays a role in the immune system. It possesses immunomodulatory, adjuvant properties and also acts as an anti-inflammatory agent. Its immunomodulatory properties can be utilized to treat several autoimmune disorders. CT can serve as one of the best model of a multifunctional protein.
Nota de preparación
delipidized
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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Certificados de análisis (COA)
Lot/Batch Number
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Los clientes también vieron
Anirudh Sethi et al.
Interface focus, 9(2), 20180076-20180076 (2019-03-08)
Cholera toxin (CT) is a secreted bacterial toxin that binds to glycoconjugate receptors on the surface of mammalian cells, enters mammalian cells through endocytic mechanisms and intoxicates mammalian cells by activating cytosolic adenylate cyclase. CT recognizes cell surface receptors through
Dazhi Jin et al.
Journal of clinical microbiology, 51(12), 3968-3974 (2013-09-21)
We report here the quantitative detection of Vibrio cholerae toxin (CT) in isolates and stool specimens by dynamic monitoring of the full course of CT-mediated cytotoxicity in a real-time cell analysis (RTCA) system. Four cell lines, including Y-1 mouse adrenal
Okoroike C Ozoemena et al.
ACS omega, 5(11), 5762-5771 (2020-04-01)
An electrochemical immunosensor for Vibrio cholerae toxin (VCT) has been developed using electrospun carbon nanofibers (CNFs) as the electrode platform. To fabricate the immunosensor, the anti-cholera toxin antibody (Ab) was covalently immobilized on the electrode platforms using the carbodiimide chemistry
A A S Baptista et al.
Poultry science, 93(1), 39-45 (2014-02-27)
This study investigated the immune response of broiler chickens with oral treatment of a Lactobacillus spp. pool (PL) associated with microencapsulated recombinant proteins flagellin (FliC) and the subunit B of cholera toxin (CTB). Immune responses were evaluated by measuring IgA
Clémence Merlen et al.
The FEBS journal, 272(17), 4385-4397 (2005-09-01)
We have defined the in vivo and in vitro metabolic fate of internalized cholera toxin (CT) in the endosomal apparatus of rat liver. In vivo, CT was internalized and accumulated in endosomes where it underwent degradation in a pH-dependent manner.
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