The antibody recognizes the human CD54 (I-CAM-1) surface antigen by flow cytometry, ELISA, immunocytochemistry, and immunohistochemistry. It inhibits human leukocyte adhesion to activated endothelial cells and stimulates homotypic aggregation of SKW3 cells. The epitope recognized by the antibody is localized in domain D2 of the CD54 molecule. 5th Workshop: code no. S 100
Immunogen
TNF-α activated human endothelial cells.
Target description
CD54 (I-CAM-1) is a glycoprotein involved in mediation of intercellular adhesion. CD54 is expressed by activated endothelial cells and is also detected on cells of many other lineages and in plasma.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4.
Preparation Note
Product filtered through a 0.2 μm filter
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