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B5144

Sigma-Aldrich

Bromelain from pineapple stem

≥4 units/mg protein, (chromatography purified)

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About This Item

Número de CAS:
37189-34-7
Comisión internacional de enzimas:
3.4.22.32 (BRENDA, IUBMB)
EC Number:
253-387-5
MDL number:
MFCD00081423
UNSPSC Code:
12352204
NACRES:
NA.54

form

lyophilized powder

Quality Level

200

specific activity

(chromatography purified)
≥4 units/mg protein

composition

Protein, 30-50% biuret

solubility

H2O: soluble 1.0 mg/mL, clear, colorless

storage temp.

−20°C

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Application

Bromelain from pineapple stem has been used as a positive control in sodium dodecyl sulfate (SDS) / polyacrylamide gel electrophoresis (PAGE) zymograms. It has also been used as a Azocasein substrate for the determination of proteolytic activity.
Bromelain may be used to inhibit the biosysnthesis of proinflammatory prostaglandins. It may be used to reduce clotting efficiency. Bromelain, from pineapple stem, has been used to make enzymatic hydrolysates of honeybee-collected pollen .

Biochem/physiol Actions

Bromelain is a cysteine endopeptidase with broad specificity for cleavage of proteins. Bromelain may be from a stem or piece of fruit. Product P3169 (pGlu-Phe-Leu-p-nitroanilide) may be used as a chromogenic substrate for the assay of bromelain.

Unit Definition

One unit will release 1.0 μmole of p-nitrophenol from N-α-Z-L-lysine p-nitrophenyl ester per min at pH 4.6 at 25 °C.

Physical form

Lyophilized powder containing mannitol and potassium phosphate buffer salts

Preparation Note

Chromatographically purified
Chromatographically purified. Dissolves in water at 1 mg/mL concentration to form a clear, colorless solution.

Substrate

Referencia del producto
Descripción
Precios

P3169

pGlu-Phe-Leu p-nitroanilide, protease substrate

C3637

Z-L-Lys-ONp hydrochloride

inhibitor

Referencia del producto
Descripción
Precios

E3132

E-64, protease inhibitor

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
0000415086
0000398549
0000415086
0000398549
0000381873

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Acidic cysteine protease inhibitors from pineapple stem.
R L Heinrikson et al.
Methods in enzymology, 45, 740-751 (1976-01-01)
Ashley G Yow et al.
Scientific reports, 13(1), 11605-11605 (2023-07-19)
Papain (aka C1A) family proteases, including bromelain enzymes, are widespread across the plant kingdom and play critical regulatory functions in protein turnover during development. The proteolytic activity exhibited by papain family proteases has led to their increased usage for a
Nora Luniak et al.
Biotechnology progress, 33(1), 54-65 (2016-11-20)
Expression of proteases in heterologous hosts remains an ambitious challenge due to severe problems associated with digestion of host proteins. On the other hand, proteases are broadly used in industrial applications and resemble promising drug candidates. Bromelain is an herbal
Azocasein substrate for determination of proteolytic activity: reexamining a traditional method using bromelain samples
Coelho DF, et al.
BioMed Research International (2016)
Grass group I pollen allergens (beta-expansins) lack proteinase activity and do not cause wall loosening via proteolysis
Li LC and Cosgrove DJ
European Journal of Biochemistry, 268(15), 4217-4226 (2001)
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