A1935

Photobiotin acetate salt

For labeling DNA probes for hybridizations

• Sinónimos: Biotin {3-[3-(4-azido-2-nitroanilino)-N-methylpropylamino]propylamide} acetate salt, N-(4-Azido-2-nitrophenyl)-N′-(3-biotinylaminopropyl)-N′-methyl-1,3-propanediamine acetate salt
• Fórmula empírica (notación de Hill): C₂₃H₃₅N₉O₄S · C₂H₄O₂
• Número de CAS: 96087-38-6
• Peso molecular: 593.70
• Número MDL: MFCD00210085
• Código UNSPSC: 12352200
• ID de la sustancia en PubChem: 24890604
• NACRES: NA.52

Propiedades

• grado: Molecular Biology; for molecular biology
• Nivel de calidad: 200
• Ensayo: ≥95%
• Formulario: film or powder
• solubilidad: H₂O: 10 mg/mL (Stable for at least 5 months if kept frozen and protected from light.)
• temp. de almacenamiento: −20°C
• cadena SMILES: CC(O)=O.CN(CCCNC(=O)CCCC[C@@H]1SCC2NC(=O)NC12)CCCNc3ccc(cc3[N+]([O-])=O)N=[N+]=[N-]
• InChI: 1S/C23H35N9O4S.C2H4O2/c1-31(12-4-10-25-17-9-8-16(29-30-24)14-19(17)32(35)36)13-5-11-26-21(33)7-3-2-6-20-22-18(15-37-20)27-23(34)28-22;1-2(3)4/h8-9,14,18,20,22,25H,2-7,10-13,15H2,1H3,(H,26,33)(H2,27,28,34);1H3,(H,3,4)/t18-,20-,22-;/m0./s1
• Clave InChI: FFBLNTOMOSLSQM-AYEYRVMASA-N

Descripción

Descripción general

Photobiotin acetate is made up of biotin, which is bound with the help of a charged linker arm to a photoreactive arylazide group.

Aplicación

Photobiotin acetate salt has been used to label DNA probes to perform DNA−DNA hybridization to determine genomic relatedness.

Acciones bioquímicas o fisiológicas

Photobiotin acetate is capable of labeling single stranded DNA and linear or supercoiled double-stranded DNA.

Photobiotin is a photo-activatable analog of biotin used primarily for labeling DNA, RNA and proteins. Probes prepared using photobiotin can detect target DNA as little as 0.5 pg.

Precaución

Light sensitive.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)