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A1420

Sigma-Aldrich

Ames′ Medium

With L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

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About This Item

UNSPSC Code:
41161501
NACRES:
NA.75

Quality Level

500

form

powder

technique(s)

cell culture | mammalian: suitable

components

glucose: 1.081 g/L (Dextro)
NaHCO3: no
L-glutamine: 0.073 g/L

shipped in

ambient

storage temp.

2-8°C

Categorías relacionadas

General description

Ames′ Medium has been formulated to support retinal tissue in relatively short-term culture. It is suitable for maintaining central nervous system tissue in vitro.

Application

Ames′ Medium has been used to maintain rat/mice/macaque monkey eye for the isolation of retina.

Quantity

Formulated to contain 8.8 grams of powder per liter of medium.

Reconstitution

Supplement with 1.9 g/L sodium bicarbonate.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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The Function of the retina and effects of drugs on it can be assessed by recording transretinal voltage across isolated retina that is perfused with physiological medium. However, building ex vivo ERG apparatus requires substantial amount of time, resources and
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Protocolos

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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