To ensure stability until the recommended retest date printed on the lot specific certificate of analysis, A1031 should be maintained at -20C after receipt. Transport conditions may differ from the recommended long-term storage conditions.
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/232/426/storage-transport-conditions-ms.pdf
A1031
αα-Amilasa from human saliva
Type XIII-A, lyophilized powder, 300-1,500 units/mg protein
Sinónimos:
1,4-α-D-glucano-glucanohidrolasa
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Número CAS:
UNSPSC Code:
12352204
eCl@ss:
32160410
EC Number:
232-565-6
NACRES:
NA.54
MDL number:
Specific activity:
300-1,500 units/mg protein
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Type XIII-A
Quality Segment
form
lyophilized powder
specific activity
300-1,500 units/mg protein
composition
Protein, 5-15% biuret
greener alternative product characteristics
Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
UniProt accession no.
greener alternative category
storage temp.
−20°C
Gene Information
human ... AMY1A(276)
General description
Estamos comprometidos a proporcionarle los productos alternativos más sostenibles , que se adhieran a uno o más de los 12 principios de la química sostenible . Este producto ha sido mejorado para mayor eficiencia energética y prevención de residuos cuando se utiliza en la investigación del etanol del almidón. Si desea más información consulte el artículo en biofiles.
La α-amilasa está compuesta por 496 aminoácidos en una sola cadena polipeptídica, que está encriptada en el cromosoma 1. Se produce en las glándulas salivares o en el páncreas. Las α-amilasas salivar y pancreática tienen secuencias primarias homólogas pero muestran patrones de hidrólisis diferentes.[1]
Application
αLa α-amilasa se utiliza para hidrolizar los enlaces α de los polisacáridos α-unidos, como el almidón y el glucógeno. El producto A1031 procede de la saliva humana, es de tipo IIA, y se suministra como polvo liofilizado. αLa α-amilasa se ha utilizado en varios estudios vegetales, como estudios del metabolismo en Arabidopsis [2]. αLa α-amilasa de la saliva humana se ha utilizado para estudiar el desarrollo de nutracéuticos, que pueden ayudar al tratamiento de la diabetes y la obesidad .
Biochem/physiol Actions
αLa α-amilasa hidroliza los enlaces α-(1,4) glucano en los polisacáridos de tres o más unidades de D-glucosa unidas por enlaces α-(1,4). Sus sustratos naturales, como el almidón y el glucógeno, se descomponen en glucosa y maltosa.[3]
Physical form
Polvo liofilizado que contiene (NH4)2SO4, citrato de sodio y cloruro de sodio.
Other Notes
Una unidad liberará 1,0 mg de maltosa del almidón en 3 minutos a pH 6,9 y a 20 °C.
Véase más información sobre enzimas para análisis de carbohidratos complejos en www.sigma-aldrich.com/enzymeexplorer
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Este artículo | |||
|---|---|---|---|
| specific activity 300-1,500 units/mg protein | specific activity 1,000-3,000 units/mg protein | specific activity ≥1,500 units/mg protein (biuret) | specific activity ~380 U/mg |
| Gene Information human ... AMY1A(276) | Gene Information human ... AMY1A(276) | Gene Information - | Gene Information - |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form powder |
| UniProt accession no. | UniProt accession no. | UniProt accession no. - | UniProt accession no. - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 100 |
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signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Clase de almacenamiento
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Can this be stored at room temperature until it is reconstituted or does it always need to be kept at -20C?
1 answer-
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Quanti mg di enzima sono contenuti in una confezione?
1 answer-
This product is sold by units and vial content will vary from lot to lot. The minimum activity of this enzyme is 300 units per milligram. The following values indicate the least amounts that will be present in each pack size:
1KU~ 3.3mg
5KU~16.6 mgThe exact specific activity will be listed on the lot specific Certificate of Analysis. Please see the link below to view a Certificate of Analysis: https://www.sigmaaldrich.com/product/sigma/a1031#product-documentation
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Considering the wide range of activity range, how can I be sure of the enzyme activity of the specific batch that I receive?
1 answer-
The protein content and enzymatic activity are determined on a lot to lot basis. Each value is reported in the specific Certificate of Analysis. The protein content range is 5 - 15% and the enzymatic activity range is 300 - 1500 units per milligram protein. A review of recent lots indicates typical protein concentrations of 7 - 13% and activities of 1350 - 1450 units per milligram protein. Please see the link below to review a sample or lot specific Certificate:
https://www.sigmaaldrich.com/product/sigma/a1031#product-documentationHelpful?
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¿Cómo se reconstituye la enzima al venir liofilizada? ¿Qué tasa de actividad tiene?
1 answer-
For assay purposes, this enzyme is reconstituted in water at 1 u/mL. This material is also soluble in 1 mM Calcium Chloride. The activity specification is 300 - 1500 U/mg. The unit per milligram value is reported in the lot-specific Certificate of Analysis and will range from 0.67 to 3.3 mg for the 1000 U pack size. Please see the link below to review a sample or lot specific Certificate:
https://www.sigmaaldrich.com/product/sigma/a1031#product-documentationHelpful?
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Hi, could you let me know what the remaining composition is when the product contains 5-15% protein? Thank you!
1 answer-
As mentioned in the 'DESCRIPTION' section, this product is a lyophilized powder containing (NH4)2SO4, sodium citrate and sodium chloride. This product is not tested for purity. However, buffer salts are expected to be the bulk of the non-protein content.
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Please let me know the storage temperature and storage period when it is dissolved in water
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The stability of this material in solution has not been investigated. However, various sources suggest that solutions may be stored in working aliquots at -20°C for several months.
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What is the recommended method for dissolving alpha amylase obtained from human saliva, specifically A1031?
1 answer-
The recommended method is to dissolve the enzyme in 1mM calcium chloride (CaCl2).
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