97875
Atto 647
BioReagent, suitable for fluorescence, ≥90% (HPCE)
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BioReagent
Quality Segment
assay
≥90% (HPCE)
form
powder
manufacturer/tradename
ATTO-TEC GmbH
transmittance
254 nm, 647 nm
fluorescence
λex 643 nm; λem 665 nm in 0.1 M phosphate pH 7.0
λ
in ethanol (with 0.1% trifluoroacetic acid)
UV absorption
λ: 645-651 nm Amax
suitability
suitable for fluorescence
storage temp.
−20°C
General description
Atto 647 is a zwitterionic dye with a net electrical charge of zero. It is a pH sensitive product. While practically stable up to pH 8.5, it slowly degrades at higher pH. Atto 647 is a new label with high molecular absorption (120,000) and quantum yield (0.20) as well as enough Stokes shift between excitation and emission maximum. Atto 647 is characterized by a high photostability and brightness. In addition, this label has good water solubility and low triplet formation ability.
Application
Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation.
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Este artículo | |||
|---|---|---|---|
| suitability suitable for fluorescence | suitability suitable for fluorescence | suitability suitable for fluorescence | suitability suitable for fluorescence |
| fluorescence λex 643 nm; λem 665 nm in 0.1 M phosphate pH 7.0 | fluorescence - | fluorescence - | fluorescence - |
| form powder | form solid | form solid | form crystals |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| assay ≥90% (HPCE) | assay - | assay ≥90% (HPLC) | assay ≥80.0% (HPCE), ≥90.0% (HPLC) |
Clase de almacenamiento
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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