ATTO 647N is a stable chemical and photochemical dye. No negative impact is expected when storing this product at -20°C with glycerol and subjecting it to multiple freeze/thaw cycles.
76508
Atto 647N Protein Labeling Kit
BioReagent, suitable for fluorescence
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NACRES:
NA.32
UNSPSC Code:
12352108
Storage temp.:
2-8°C
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Servicio técnico
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Permítanos ayudarleproduct line
BioReagent
Quality Segment
manufacturer/tradename
ATTO-TEC GmbH
fluorescence
λex 647 nm; λem 661 nm in 0.1 M phosphate buffer, pH 7.0 (recommended)
suitability
suitable for fluorescence
storage temp.
2-8°C
General description
This kit contains sufficient amounts of reactive dye, buffers and protein purification sets for performing five labeling reactions (1 mg protein each) and for the subsequent purification of the labeled protein.
Legal Information
This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.
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Este artículo | |||
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| manufacturer/tradename ATTO-TEC GmbH | manufacturer/tradename ATTO-TEC GmbH | manufacturer/tradename ATTO-TEC GmbH | manufacturer/tradename ATTO-TEC GmbH |
| fluorescence λex 647 nm; λem 661 nm in 0.1 M phosphate buffer, pH 7.0 (recommended) | fluorescence λex 633 nm; λem 661 nm in 0.1 M phosphate buffer, pH 7.0 (recommended) | fluorescence λex 544 nm; λem 576 nm in 0.1 M phosphate buffer, pH 7.0 (recommended) | fluorescence λex 488 nm; λem 520 nm in 0.1 M phosphate buffer, pH 7.0 (recommended) |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| suitability suitable for fluorescence | suitability suitable for fluorescence | suitability suitable for fluorescence | suitability suitable for fluorescence |
| product line BioReagent | product line BioReagent | product line BioReagent | product line BioReagent |
Clase de almacenamiento
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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Can we freeze/defreeAfter marking and storing the product at -20°C with glycerol, does the marking remain stable after multiple freeze/thaw cycles?
1 answer-
Helpful?
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Is there an optimal volume of protein at c=2mg/ml (e.g. is it better to have 1 mg in 0.5ml PBS or 2mg in 1ml PBS). Incubation of 2h is performed on RT or +4C?
1 answer-
Per the product technical bulletin, the labeling reaction is sufficient for 1 mg of protein at a concentration of 2–10 mg/mL, and the two-hour incubation step is at room temperature.
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Will this kit efficiently label protein on a much smaller scale of < 20ug of protein or at concentration below 2 mg/ml?
1 answer-
The dye should be reconstituted in 20 ul of DMSO and the total volume added to 1 mg of the antibody (at 2-10 mg/ml). If the amount of antibody is less, it needs to be at 2 mg/ml, and the amount of dye added to the antibody solution should be proportionally reduced. For example, if there is 200 ug of antibody (at 2 mg/ml), 4 ul of the DMSO solution of the dye should be added to the antibody. It's important for the user to use very accurate pipettes to avoid adding too much dye to the antibody, which could result in the labeling of the antibody in the antigen-binding site. If less protein is used, the amount of dye can be reduced. There is always an excess of dye to ensure labeling efficiency, and a part of the dye will be separated (as unbound dye) by a purification step afterward. The proteins need to have a minimum concentration due to the strong hydrolysis tendency of the NHS-functionality. If the protein solution is too dilute, the dye will be hydrolyzed before it reaches an amine-group of a protein.
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