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53608

Sigma-Aldrich

Biotin (5-fluorescein) conjugate

BioReagent, suitable for fluorescence, ≥90% (HPCE)

Sinónimos:

N-(Biotinylamidoethyl)-fluorescein-5-carboxamide, N-(Fluorescein-5-carbamidoethyl)biotinamide

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About This Item

Fórmula empírica (notación de Hill):
C33H32N4O8S
Número de CAS:
957494-27-8
Peso molecular:
644.69
MDL number:
MFCD03791084
UNSPSC Code:
12352108
eCl@ss:
34058011
PubChem Substance ID:
57651424
NACRES:
NA.32

product line

BioReagent

Quality Level

100

assay

≥90% (HPCE)

solubility

DMSO: soluble

fluorescence

λex 490 nm; λem 524 nm in 0.1 M Tris pH 8.0

suitability

suitable for fluorescence

storage temp.

−20°C

SMILES string

[H][C@]12CS[C@@H](CCCCC(=O)NCCNC(=O)c3ccc(c(c3)C(O)=O)C4=C5C=CC(=O)C=C5Oc6cc(O)ccc46)[C@@]1([H])NC(=O)N2

InChI

1S/C33H32N4O8S/c38-18-6-9-21-25(14-18)45-26-15-19(39)7-10-22(26)29(21)20-8-5-17(13-23(20)32(42)43)31(41)35-12-11-34-28(40)4-2-1-3-27-30-24(16-46-27)36-33(44)37-30/h5-10,13-15,24,27,30,38H,1-4,11-12,16H2,(H,34,40)(H,35,41)(H,42,43)(H2,36,37,44)/t24-,27-,30-/m0/s1

InChI key

DFUFXKZUEOKPSD-LFERIPGTSA-N

Application

Biotin (5-fluorescein) conjugate is a reagent that may be used in situations similar to fluorescein isothiocyanate staining to label avidin/streptavidin conjugated molecules such as antibodies with the fluorophore 5-fluorescein.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Other Notes

Biotin-fluorescein conjugate for the fluorometric, accurate measurement of avidin and streptavidin.
Often referred to as Biotin (4-fluorescein) conjugate.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Ann L Baldwin et al.
Journal of the American Association for Laboratory Animal Science : JAALAS, 46(1), 58-65 (2007-01-06)
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H J Gruber et al.
Biochimica et biophysica acta, 1381(2), 203-212 (1998-08-01)
A new fluorimetric assay is presented for the specific and reliable quantitation of >/=2 nM avidin and streptavidin. The assay is based on pronounced changes in the fluorescence properties of commercial fluorescein-biotin, or of a newly synthesized biotin-poly(ethylene glycol)-pyrene conjugate
Damien D Pearse et al.
The European journal of neuroscience, 20(12), 3387-3396 (2004-12-22)
Microglia respond in a stereotypical pattern to a diverse array of pathological states. These changes are coupled to morphological and immunophenotypical alterations and the release of a variety of reactive species, trophic factors and cytokines that modify both microglia and
G. Kada et al.
Biochimica et Biophysica Acta, 1427, 33-33 (1998)

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