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35845

DCFHDA

BioReagent, suitable for fluorescence, ≥95% (HPLC)

Sinónimos:

2′,7′-Dichlorofluorescein diacetate, Diacetyldichlorofluorescein

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Fórmula empírica (notación de Hill):
C24H14Cl2O7
Número CAS:
2044-85-1
Peso molecular:
485.27
UNSPSC Code:
12352108
NACRES:
NA.32
PubChem Substance ID:
57649438
MDL number:
MFCD00037501
Beilstein/REAXYS Number:
68488
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Quality Segment

100

description

suitable as a fluorogenic substrate for esterases

product line

BioReagent

assay

≥95% (HPLC)

form

powder or crystals

mol wt

485.27 g/mol

color

faint red, white to faint brown

mp

232-234 °C (lit.)

solubility

DMSO: soluble

fluorescence

λex 504 nm; λem 524 nm in 0.1 M Tris pH 8.0 (esterase)

suitability

suitable for fluorescence

storage temp.

2-8°C

SMILES string

CC(=O)Oc1cc2Oc3cc(OC(C)=O)c(Cl)cc3C4(OC(=O)c5ccccc45)c2cc1Cl

InChI

1S/C24H14Cl2O7/c1-11(27)30-21-9-19-15(7-17(21)25)24(14-6-4-3-5-13(14)23(29)33-24)16-8-18(26)22(31-12(2)28)10-20(16)32-19/h3-10H,1-2H3

InChI key

VQVUBYASAICPFU-UHFFFAOYSA-N

General description

2′,7′-Dichlorofluorescein diacetate, also known as DCFHDA or Dcfh-DA, is a sensitive cell-permeable redox probe. The lipophilic non-fluorescent Dcfh-DA readily crosses the cell membrane through passive diffusion followed by deacetylation. The deacylated product is an oxidant sensitive 2′,7′-dichlorofluorescein (DCHF). DCHF is oxidized later to form highly fluorescent DCF, which is measured.[1]

Application

2′,7′-Dichlorofluorescein diacetate is predominantly used to detect reactive oxygen (ROS) intermediates and quantify the overall oxidative stress in cells.[1]

Dcfh-DA is used in the quantitative assay to measure oxidative stress in nanoparticle-treated cells.[2]

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Este artículo
2188435848287810
DCFHDA BioReagent, suitable for fluorescence, ≥95% (HPLC)

Sigma-Aldrich

35845

DCFHDA

5(6)-Carboxy-2′,7′-dichlorofluorescein diacetate BioReagent, suitable for fluorescence, ≥85% (HPCE)

Sigma-Aldrich

21884

5(6)-Carboxy-2′,7′-dichlorofluorescein diacetate

2′,7′-Dichlorofluorescein BioReagent, suitable for fluorescence, ≥90% (T)

Sigma-Aldrich

35848

2′,7′-Dichlorofluorescein

2′,7′-Dichlorofluorescin Diacetate Cell-permeable fluorogenic probe that is useful for the detection of reactive oxygen species (ROS) and nitric oxide (•NO) and for the determination of the degree of overall oxidative stress.

Sigma-Aldrich

287810

2′,7′-Dichlorofluorescin Diacetate

suitability

suitable for fluorescence

suitability

suitable for fluorescence

suitability

suitable for fluorescence

suitability

-

fluorescence

λex 504 nm; λem 524 nm in 0.1 M Tris pH 8.0 (esterase)

fluorescence

λex 470 nm; λem 529 nm in 0.1 M Tris pH 8.0 (esterase)

fluorescence

λex 504 nm; λem 529 nm in 0.1 M Tris pH 8.0

fluorescence

-

color

faint red, white to faint brown

color

-

color

-

color

-

form

powder or crystals

form

-

form

solid

form

crystalline solid

solubility

DMSO: soluble

solubility

DMF: soluble, DMSO: soluble, methanol: soluble

solubility

-

solubility

DMSO: 5 mg/mL

assay

≥95% (HPLC)

assay

≥85% (HPCE)

assay

≥90% (T)

assay

≥94% (HPLC)

Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificados de análisis (COA)

Lot/Batch Number
BCCN8736
BCCN2492
BCCL7891
BCCM2610
BCCL2220

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Número de artículo de comercio global

SKUGTIN
35845-5G04061831812824
35845-1G04061835504411

Questions

1–8 of 8 Questions  

  1. Can I get the protocol? Thank you

    1 answer

    1. A protocol is not available for this compound.

      Please see the link below to review a publication that may be helpful.
      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7712457/

      Helpful?

  2. What is the shelf life of this product, as a powder, and once dissolved?

    1 answer

    1. This product is not assigned an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment. For more information you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.

      Stock solutions of this item in organic solvents are stable for at least 6 months if stored at -20°C.

      Helpful?

  3. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  4. What are the optical properties of the hydrolysis product of 2´,7´-Dichlorofluorescein diacetate, Product 35845?

    1 answer

    1. Excitation maximum is at 504 nm; emission maximum is at 529 nm (in 0.1 M Tris pH 8.0 following treatment with an esterase).

      Helpful?

  5. Can Product 35845, 2´,7´-Dichlorofluorescein diacetate be used for ROS (Reactive Oxygen Species) detection?

    1 answer

    1. Yes, Product 35845, 2´,7´-Dichlorofluorescein diacetate can be used for ROS detection by FACS analysis.

      Helpful?

  6. In what solvents is 2´,7´-´Dichlorofluorescein diacetate, Product 35845, soluble?

    1 answer

    1. It is soluble in DMF, ethanol (2 mg/mL), DMSO (10 mg/mL).

      Helpful?

  7. What are the recommended storage conditions for 2´\,7´-Dichlorofluorescein diacetate, Product 35845?

    1 answer

    1. Product no. 35845 should be stored in a refrigerator or coldroom at 2-8°C.

      Helpful?

  8. What is required to get 2´,7´-Dichlorofluorescein diacetate, Product 35845, to fluoresce?

    1 answer

    1. 2',7'-dichlorofluorescein diacetate fluoresces only after cleavage of the acetyl groups.

      Helpful?

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