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60983

Millipore

Kovac′s reagent for indoles

suitable for microbiology

Sinónimos:

4-(Dimethylamino)benzaldehyde solution, Indole reagent according to Kovac

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About This Item

Fórmula empírica (notación de Hill):
C9H11NO
Número de CAS:
100-10-7
Peso molecular:
149.19
Beilstein:
4132845
Número MDL:
MFCD00003381
Código UNSPSC:
41171621
ID de la sustancia en PubChem:
329759335
NACRES:
NA.85

caducidad

limited shelf life, expiry date on the label

Nivel de calidad

100

composición

n-butanol
4-dimethylaminobenzaldehyde
hydrochloric acid

técnicas

microbe id | specific enzyme detection: suitable

índice de refracción

n20/D 1.400-1.420

aplicaciones

agriculture
clinical testing
environmental
food and beverages
microbiology

temp. de almacenamiento

2-8°C

idoneidad

Escherichia coli
coliforms

cadena SMILES

[H]C(=O)c1ccc(cc1)N(C)C

InChI

1S/C9H11NO/c1-10(2)9-5-3-8(7-11)4-6-9/h3-7H,1-2H3

Clave InChI

BGNGWHSBYQYVRX-UHFFFAOYSA-N

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Descripción general

Kovac′s reagent for indoles is a solution of 4-(dimethylamino)benzaldehyde and hydrochloric acid in n-butanol.

Aplicación

Kovac′s reagent for indoles has been used for enumerating E. coli O157:H7 in ground beef samples in different scenarios of stored, thawed, and as cooked patty samples. It has also been used for E coli enumeration in dewatered blackwater.

Acciones bioquímicas o fisiológicas

Kovac′s reagent detects the indole and aids in identifying the indole-positive and indole-negative microorganisms. E. coli can split tryptophan into indole and α-aminopropionic acid in the presence of the enzyme tryptophanase. p-Aminobenzaldehyde present in the reagent reacts with indole to form a cherry-red complex, that is soluble in alcohol, ether, and chloroform. It is recommended to use glucose free media and peptone with a high tryptophan content for the test.

Palabra de señalización

Danger

Clasificaciones de peligro

Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3

Órganos de actuación

Central nervous system, Respiratory system

Código de clase de almacenamiento

3 - Flammable liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

96.8 °F

Punto de inflamabilidad (°C)

36 °C

Equipo de protección personal

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

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Certificados de análisis (COA)

Lot/Batch Number
BCCL0925
BCCK8015
BCCM2245
BCCJ6939
BCCG3903

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Stavros G Manios et al.
Meat science, 101, 25-32 (2014-12-03)
The effect of common handling practices (i.e., freezing, thawing and cooking) of beef patties on the survival of Salmonella spp. and Escherichia coli O157:H7, was evaluated. Inoculated ground beef was stored at -22 °C for 5 and 75 days. After
Ragnhildur Gunnarsdóttir et al.
Environmental science & technology, 46(22), 12408-12416 (2012-11-02)
Wastewater treatment in many Arctic regions is inadequate, even nonexisting. Natural freezing of wastewater in those areas may be beneficial for reduction of microorganisms. The aim of this study was to investigate the effect of long-term freezing, and repeated freezing
G L Lombard et al.
Journal of clinical microbiology, 18(3), 609-613 (1983-09-01)
Three reagents for detecting indole, Kovac, Ehrlich, and p-dimethylaminocinnamaldehyde (DMCA), were evaluated with commercial microtest systems for characterizing and identifying anaerobic bacteria. The DMCA reagent, the most sensitive of the three reagents, gave a positive reaction with 445 of 449
Jun-Min Guo et al.
Journal of agricultural and food chemistry, 58(11), 6556-6561 (2010-05-15)
A simple colorimetric method for the differentiation of indoleacetic acid (IAA) and indolebutyric acid (IBA) in plant samples is described. The color change is based upon the reaction between the auxins and p-(dimethylamino)benzaldehyde (PDAB, Ehrlich reagent) following the electrophilic substitution
Spectrophotometric determination of certain cephalosporins through oxidation with cerium(IV) and 1-chlorobenzotriazole.
M M Ayad et al.
Journal of pharmaceutical and biomedical analysis, 20(3), 557-564 (2000-03-04)
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