11088939001

Tumor Necrosis Factor-α, human (hTNF-α)

recombinant (yeast)

• Sinónimos: hTNF, human necrosis factor
• Código UNSPSC: 12352207

Propiedades

• origen biológico: human
• Nivel de calidad: 100
• recombinante: expressed in yeast
• Ensayo: ≥95% (SDS-PAGE)
• Formulario: solution
• potencia: ≤0.01 ng/mL EC₅₀
• actividad específica: >1*10^8 U/mg
• mol peso: 17,000 Da
• envase: pkg of 1,000,000 U (10 μg, 1ml)
• fabricante / nombre comercial: Roche
• condiciones de almacenamiento: avoid repeated freeze/thaw cycles
• impurezas: <0.1 EU/μgtested (LAL test)
• Nº de acceso UniProt: Q9UBM5
• temp. de almacenamiento: −20°C
• Información sobre el gen: human ... TNF(7124)

Descripción

Descripción general

>1 x 10^⊥8 U/mg, <0.01 ng/ml (hTNF-a, NIBSC, interim standard, 87/650), at least the same specific activity (EC50) compared to the indicated standard is guaranteed.

Especificidad

Human hTNF-α is effective on human and mouse cells.

Species specificity: human, mouse

Aplicación

Tumor Necrosis Factor-α (hTNF-α)

• Shows cytolytic and/or cytostatic activity on a variety of transformed cell lines.
• Has activating and growth stimulating activities on a variety of normal cells.
• Has antiviral activity on many cell types in vitro.
• Can cause tumor necrosis of certain tumors in vivo.

Acciones bioquímicas o fisiológicas

Receptor p55 and p75, belonging to the TNFR gene family, mediate the biological activity of TNF-a, and are co-expressed on the surface of various cell types. The pleiotropic action of TNF-a is initiated by binding to either of the two receptors.

Calidad

Purity: >95% (SDS-PAGE)
Endotoxin level: <0.1 EU/μg (LAL-test), <10 EU/ml (LAL-test)

Secuencia

Chain Length 157 AA

One polypeptide chain (157 amino acids), identical to natural hTNF-α.

Definición de unidad

EC₅₀ definition: The amount of hTNF-α that is required to mediate half-maximal cytotoxicity (MTT cleavage) with WEHI 164 cells in the presence of actinomycin D (1 unit equals ≤0.01 ng/ml).

Forma física

Solution, filtered through 0.2 μm pore size membrane.

Nota de preparación

Working concentration: 1 ng/ml
For complete cell lysis of sensitive cell lines about 1 ng/ml is recommended.
Working solution: Dilute the concentrated hTNF-α solution (10 μg/ml) with PBS or culture medium containing BSA (or HSA), 1 mg/ml (0.1%) or serum 1 to 10%.
Storage conditions (working solution): -15 to -25 °C
It is recommended to store the solution in aliquots at -15 to -25 °C.
Note: Avoid repeated freezing and thawing.

Otras notas

For life science research only. Not for use in diagnostic procedures. 1 EU corresponds to 0.1 ng

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): nwg
• Punto de inflamabilidad (°F): does not flash
• Punto de inflamabilidad (°C): does not flash